Antioxidant-guided isolation and mass spectrometric identification of the major polyphenols in barley (Hordeum vulgare) grain
Antioxidant-guided isolation and mass spectrometric identification of the major polyphenols in barley (Hordeum vulgare) grain
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DOI：
10.1016/j.foodchem.2016.04.098
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发表时间：
2016-11-01
期刊：
影响因子：
8.8
通讯作者：
Hossain, Mohammad B.
中科院分区：
文献类型：
--
作者：
Gangopadhyay, Nirupama;Rai, Dilip K.;Hossain, Mohammad B.
在本研究中，个人/类多酚在大麦的相对贡献，其抗氧化性能，进行了评估。快速色谱法被用来fractionated爱尔兰大麦品种'Irina'的总多酚提取物，并确定使用总酚含量和三个体外抗氧化剂测定：DPPH，FRAP，和ORAC具有最高的抗氧化性能的馏分。黄烷醇（儿茶素、原花青素B、原飞燕草素B、原花青素C）和一种新的取代黄烷醇（儿茶素二己糖苷，C27 H33 O 16，m/z 613.17）被鉴定为具有最高抗氧化能力的组分。在鉴定其他活性级分中的酚类物质后，观察到的大麦提取物的抗氧化能力的最有效贡献者的顺序为黄烷醇>黄酮醇（槲皮素）>羟基肉桂酸（阿魏酸、咖啡酸、香豆酸）。总提取物中含量最高的多酚是阿魏酸（277.7 μ g/g dw大麦），其次是矢车菊苷配质B（73.7 μ g/g dw大麦）。(C)2016爱思唯尔有限公司版权所有。
In the present study, the relative contribution of individual/classes of polyphenols in barley, to its antioxidant properties, was evaluated. Flash chromatography was used to fractionate the total polyphenol extract of Irish barley cultivar 'Irina', and fractions with highest antioxidant properties were identified using total phenolic content and three in vitro antioxidant assays: DPPH, FRAP, and ORAC. Flavanols (catechin, procyanidin B, prodelphinidin B, procyanidin C) and a novel substituted flavanol (catechin dihexoside, C27H33O16, m/z 613.17), were identified as constituents of the fraction with highest antioxidant capacity. Upon identification of phenolics in the other active fractions, the order of most potent contributors to observed antioxidant capacity of barley extract were, flavanols > flavonols (quercetin) > hydroxycinnamic acids (ferulic, caffeic, coumaric acids). The most abundant polyphenol in the overall extract was ferulic acid (277.7 mu g/g dw barley), followed by procyanidin B (73.7 mu g/g dw barley). (C) 2016 Elsevier Ltd. All rights reserved.