Anti-TMV Effects of Amaryllidaceae Alkaloids Isolated from the Bulbs of Lycoris radiata and Lycoricidine Derivatives.
Anti-TMV Effects of Amaryllidaceae Alkaloids Isolated from the Bulbs of Lycoris radiata and Lycoricidine Derivatives.
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从石蒜球茎中分离出的石蒜科生物碱和石蒜素衍生物的抗 TMV 作用
DOI：
10.1007/s13659-018-0163-0
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发表时间：
2018-06
影响因子：
4.7
通讯作者：
Li SL
中科院分区：
文献类型：
--
作者：
Yang DQ;Chen ZR;Chen DZ;Hao XJ;Li SL
从石蒜（Lycorisradiata）鳞茎中分离得到15个石蒜科生物碱。采用常规半叶法对部分化合物和力可利西啶衍生物进行了抗烟草花叶病毒活性筛选。用Western-blot和RT-PCR方法测定了石蒜碱衍生物对TMV系统感染的抑制作用。供试化合物表现出中等的灭活效果，而力可西定衍生物表现出良好的保护效果。化合物L1（N-甲基-2，3，4-三甲基环可利西啶）和L3（N-甲基-2-甲氧基-3，4-丙酮基环可利西啶）的保护性抑制活性分别为60.8%和62.0%，与阳性对照宁南霉素（66.4%）相近。RT-PCR和Western-blot分析表明，化合物L1、L3、L5（N-烯丙基-2，3，4-三烯丙氧基甘草定）具有一定的抗病毒活性，抑制TMV CP基因的复制和蛋白表达。另外，抗病酶活性测定证实，化合物L1可提高PAL、POD、SOD活性，提高烟草抗病性。
Fifteen known amaryllidaceae alkaloids were isolated from the bulbs of Lycoris radiata. Some of the compounds and lycoricidine derivatives had been screened for the activities against tobacco mosaic virus (TMV) by the conventional half-leaf method. Lycoricidine derivatives were also carried out the assay of effect on systemic infection of TMV by western-blot and RT-PCR analysis. The tested compounds showed moderate inactivation effect, whereas the lycoricidine derivatives showed good protective effect. The protective inhibitory activity of compounds L1 (N-methyl-2,3,4-trimethoxylycoricidine) (60.8%) and L3 (N-methyl-2-methoxy-3,4-acetonidelycoricidine) (62.0%) was almost similar to the positive control, Ningnanmycin (66.4%). RT-PCR and Western-blot analysis displayed that compounds L1, L3, L5 (N-allyl-2,3,4-triallyloxylycoricidine) exhibited antiviral activity, which was evidenced by reducing TMV-CP gene replication and TMV-CP protein expression. Additionally, defensive enzyme activities confirmed that compound L1 could increase the activity of PAL, POD, SOD to improve disease resistance of tobacco.