Bioactive Extract from Moringa oleifera Inhibits the Pro-inflammatory Mediators in Lipopolysaccharide Stimulated Macrophages.
Bioactive Extract from Moringa oleifera Inhibits the Pro-inflammatory Mediators in Lipopolysaccharide Stimulated Macrophages.
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DOI：
10.4103/0973-1296.172961
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发表时间：
2015-10
影响因子：
0.7
通讯作者：
Fakurazi S
中科院分区：
文献类型：
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作者：
Fard MT;Arulselvan P;Karthivashan G;Adam SK;Fakurazi S
炎症是一种众所周知的生理反应，可以保护身体免受感染，恢复组织损伤。然而，慢性炎症可引发各种炎症性疾病/紊乱。辣木是一种在大多数热带国家广泛种植的植物，传统上被认为具有多种药用价值。本研究的目的是研究油茶提取物对内毒素刺激的巨噬细胞的抗炎作用。通过抑制Griess反应中一氧化氮(NO)的产生和巨噬细胞中促炎介质的表达来评价油茶叶水乙醇生物活性提取物的抗炎作用。有趣的是，我们发现油菜水乙醇活性叶提取物显著抑制NO的产生和其他炎症标志物的分泌，如前列腺素E2、肿瘤坏死因子α、白介素6和白介素1β。同时，该生物活性提取物以剂量依赖的方式诱导IL-10的产生。此外，油茶叶水乙醇提取物还能有效抑制脂多糖诱导RAW264.7巨噬细胞活化的B细胞p65中炎症标志物诱导型一氧化氮合酶、环氧合酶-2和核因子-kappa轻链增强子的蛋白表达，并呈剂量依赖关系。这些发现支持了传统上使用油菜作为治疗炎症相关疾病/紊乱的有效方法。辣木醇提物能有效抑制脂多糖诱导的炎症模型中NO的产生。油茶粗提物成功地调节了脂多糖刺激的巨噬细胞产生促炎介质。油茶提取物抑制脂多糖刺激的巨噬细胞炎症介质的表达。
Inflammation is a well-known physiological response to protect the body against infection and restore tissue injury. Nevertheless, the chronic inflammation can trigger various inflammatory associated diseases/disorder. Moringa oleifera is a widely grown plant in most tropical countries and it has been recognized traditionally for several medicinal benefits. The objective of this study was to investigate the anti-inflammatory properties of M. oleifera extract on lipopolysaccharide (LPS) - stimulated macrophages. The anti-inflammatory effect of M. oleifera hydroethanolic bioactive leaves extracts was evaluated by assessing the inhibition of nitric oxide (NO) production during Griess reaction and the expression of pro-inflammatory mediators in macrophages. Interestingly, we found that M. oleifera hydroethanolic bioactive leaves extract significantly inhibited the secretion of NO production and other inflammatory markers such as prostaglandin E2, tumor necrosis factor alpha, interleukin (IL)-6, and IL-1β. Meanwhile, the bioactive extract has induced the production of IL-10 in a dose-dependent manner. In addition, M. oleifera hydroethanolic bioactive leaves extract effectively suppressed the protein expression of inflammatory markers inducible NO synthase, cyclooxygenase-2, and nuclear factor kappa-light-chain-enhancer of activated B-cells p65 in LPS-induced RAW264.7 macrophages in a dose-dependent manner. These findings support the traditional use of M. oleifera plant as an effective treatment for inflammation associated diseases/disorders. Hydroethanolic extracts of Moringa oleifera effectively inhibit the NO production in LPS induced inflammatory model. M. oleifera crude extracts successfully modulate the production of pro-inflammatory mediators in LPS stimulated macrophages. M. oleifera extracts suppressed the expression of inflammatory mediators in LPS stimulated macrophages.