Deletion mapping reveals two regions of chromosome 8 allele loss in colorectal carcinomas.
Deletion mapping reveals two regions of chromosome 8 allele loss in colorectal carcinomas.
复制标题
缺失图谱揭示了结直肠癌中 8 号染色体等位基因的两个区域丢失。
DOI：
10.1002/gcc.2870100102
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Westbrook,CA
中科院分区：
文献类型：
--
作者：
Yaremko,ML;Wasylyshyn,ML;Paulus,KL;Michelassi,F;Westbrook,CA
结直肠癌的发生与显性癌基因和肿瘤抑制基因的遗传变化积累有关。尽管至少有四种不同的基因参与了这一过程，但从基因组其他区域检测到的等位基因丢失表明，还有其他基因参与了这一过程。8号染色体的短臂就是这些区域之一；杂合性丧失的发生率在30%到50%之间。为了确定含有推定肿瘤抑制基因的共同缺失区域，我们使用Southern blot分析和一组多态性探针分析了一系列87例肿瘤中8号染色体短臂不同区域的等位基因缺失。我们在33%的病例中发现等位基因丢失，其中涉及两个独立的区域，一个在染色体的p端区域，8p23.1 - pter， 45%的信息性病例显示等位基因丢失，另一个在中p区域，8p21, 31%的病例显示等位基因丢失。没有肿瘤显示这两个区域的杂合性缺失。这些发现表明，在8号染色体短臂上存在两个与结直肠癌发生有关的离散基因。基因：1 - 6（1994）。©1994 Wiley‐Liss, Inc。
Colorectal carcinogenesis is associated with the accumulation of genetic changes involving both dominant oncogenes and tumor suppressor genes. Although at least four different genes have been implicated in the process, the detection of allele loss from other regions of the genome suggests the involvement of additional genes. The short arm of chromosome 8 is one of these regions; loss of heterozygosity occurs at rates ranging from 30 to 50%. To define the region of common deletion containing the putative tumor suppressor gene, we analyzed a series of 87 carcinomas for allele loss in different regions of the short arm of chromosome 8 by using Southern blot analysis and a panel of polymorphic probes. We found allele loss in 33% of our cases, which involves two separate regions, one in the p‐terminal region of the chromosome, 8p23.1 ‐pter, where 45% of informative cases demonstrated loss, and the other in the mid‐p region, at 8p21, where 31% of cases showed allele loss. No tumors showed loss of heterozygosity for both regions. These findings suggest the presence of two discrete genes related to colorectal carcinogenesis on the short arm of chromosome 8. Genes Chrom Cancer 10:1–6 (1994). © 1994 Wiley‐Liss, Inc.
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影响因子：
8.8
作者：
Rees M;Leigh SE;Delhanty JD;Jass JR
通讯作者：
Jass JR
影响因子：
56.9
作者：
NISHISHO, I;NAKAMURA, Y;VOGELSTEIN, B
通讯作者：
VOGELSTEIN, B
缺失%20in%20the%20short%20arm%20of%20染色体%208%20are%20present%20in%20up%20to%2090%%20of%20 human%20colorectal%20cancer%20cell%20lines
影响因子：
3.5
作者：
Kai Van Der Bosch;Ilona Becker;L. Savelyeva;S. Brüderlein;Peter M. Schlag;M. Schwab
通讯作者：
M. Schwab
一个新的 RFLP 位点 D8S163 映射到人类染色体 8pter-8p22。
影响因子：
14.9
作者：
K. Kaneko;M. Wagner;D. Wells;Hajime Tanaka;T. Miyatake;S. Tsuji
通讯作者：
S. Tsuji
影响因子：
1.7
作者：
J. Hamerton;P. Cook
通讯作者：
P. Cook