EFFICACY OF PHOSPHOLIPID ANALYSIS IN DETERMINING MICROBIAL BIOMASS IN SEDIMENTS
EFFICACY OF PHOSPHOLIPID ANALYSIS IN DETERMINING MICROBIAL BIOMASS IN SEDIMENTS
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DOI：
10.1128/aem.55.11.2888-2893.1989
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发表时间：
1989-11-01
影响因子：
4.4
通讯作者：
WATLING, L
中科院分区：
文献类型：
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作者：
FINDLAY, RH;KING, GM;WATLING, L
脂质结合磷酸盐分析的改进产生了一种用于测定沉积物中微生物生物量的简化且灵敏的方法。通过使用染料孔雀石绿绿色，其在低pH下与磷钼酸盐络合时具有高消光系数（在610 nm处），灵敏度比以前的方法提高。使用过硫酸盐氧化技术从脂质中释放磷酸盐，相对于传统的高氯酸二氢氧化法，增加了该方法的简单性和安全性。改进的方法是准确的（产生定量回收的细胞添加到沉积物）和精密度（细胞和沉积物的变异系数小于5%）。与落射荧光技术的比较表明，脂质结合磷酸盐的分析更快速，更不繁琐，可以成功地应用于更广泛的沉积物类型。一个估计的脂质结合的磷酸盐到碳的转换因子的基础上，从沉积物中的各种富集文化表明，以前的纯文化的因素可能太低了。
Improvements of the analysis of lipid-bound phosphates resulted in a simplified and sensitive method for determining microbial biomass in sediments. Sensitivity was enhanced over previous methods by use of a dye, malachite green, which when complexed with phosphomolybdate at low pH has a high extinction coefficient (at 610 nm). The use of a persulfate oxidation technique to liberate phosphate from lipids increased the simplicity and safety of the method relative to the traditional perchloric acid digestions. The modified method was both accurate (yielding quantitative recoveries of cells added to sediments) and precise (coefficient of variation of less than 5% for cells and sediments). A comparison with an epifluorescence technique indicated that the analysis of lipid-bound phosphate was more rapid and less tedious and could be successfully applied to a wider variety of sediment types. An estimate of the lipid-bound phosphate-to-carbon conversion factor based on a diverse enrichment culture from sediments suggested that previous factors for pure cultures may have been too low.