Rice-Spikelet-like Copper Oxide Decorated with Platinum Stranded in the CNT Network for Electrochemical In Vitro Detection of Serotonin
Rice-Spikelet-like Copper Oxide Decorated with Platinum Stranded in the CNT Network for Electrochemical In Vitro Detection of Serotonin
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DOI：
10.1021/acsami.0c20645
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发表时间：
2021-01-26
影响因子：
9.5
通讯作者：
Liu, Hongfang
中科院分区：
文献类型：
--
作者：
Ashraf, Ghazala;Asif, Muhammad;Liu, Hongfang
5-羟色胺（ST）是公认的第三大内源性胃肠神经递质，对ST的特异性监测引起了治疗和生物科学的极大兴趣。因此，非常需要开发一种灵敏且低成本的传感平台，用于检测生物基质中的临床相关ST水平。在此，我们开发了一种简单的两步法，用于具有Cu 2 O金属氧化物（MO）结合的CNT核心的超灵敏电化学（EC）传感器，该CNT核心已进一步沉积有过渡量的铂纳米颗粒（Pt NPs）。我们提出，第一次，通过电化学置换法在（CNTs-Cu 2 O-CuO）纳米金属复合材料上沉积Pt NPs，其中铜不仅作为还原剂，而且作为牺牲模板。已经评估了所制造的EC传感平台的电催化能力，用于敏感地检测ST作为早期疾病诊断中的熟练生物标志物。提高的活性表面积，显着的导电性，铂纳米粒子上的CNTs-Cu 2 O-CuO纳米金属片，快速的电子转移，和混合价态的铜诱导的极化效应的协同作用，提高了电极-分析物交界处的氧化还原过程。CNTs-Cu 2 O-CuO @ Pt修饰电极在3 nM（S/N = 3）的低检测限、宽线性浓度范围、重现性和令人难以置信的耐用性方面展现了对ST氧化的出色电催化能力。由于惊人的熟练程度，提出的EC传感器的基础上CNTs-Cu 2 O-CuO @Pt异质结构已被应用于ST检测在生物流体和实时跟踪ST流出释放从各种细胞系作为早期疾病诊断方法。
The specific monitoring of serotonin (ST) has provoked massive interest in therapeutic and biological science since it has been recognized as the third most significant endogenous gastrointestinal neurotransmitter. Hence, there is a great need to develop a sensitive and low-cost sensing platform for the detection of a clinically relevant ST level in biological matrices. Herein, we develop a simple two-step approach for an ultrasensitive electrochemical (EC) sensor with the Cu2O metal oxide (MO)-incorporated CNT core that has been further deposited with a transitional amount of platinum nanoparticles (Pt NPs). We presented, for the first time, the deposition of Pt NPs on the (CNTs-Cu2O-CuO) nanopetal composite via the galvanic replacement method, where copper not only acts as a reductant but a sacrificial template as well. The electrocatalytic aptitude of the fabricated EC sensing platform has been assessed for the sensitive detection of ST as a proficient biomarker in early disease diagnostics. The synergy of improved active surface area, remarkable conductivity, polarization effect induced by Pt NPs on CNTs-Cu2O-CuO nanopetals, fast electron transfer, and mixed-valence states of copper boost up the redox processes at the electrode-analyte junction. The CNTs-Cu2O-CuO@Pt-modified electrode has unveiled outstanding electrocatalytic capabilities toward ST oxidation in terms of a low detection limit of 3 nM (S/N = 3), wide linear concentration range, reproducibility, and incredible durability. Owing to the amazing proficiency, the proposed EC sensor based on the CNTs-Cu2O-CuO@Pt heterostructure has been applied for ST detection in biotic fluids and real-time tracking of ST efflux released from various cell lines as early disease diagnostic approaches.