High-throughput binary vectors for plant gene function analysis
High-throughput binary vectors for plant gene function analysis
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DOI：
10.1111/j.1744-7909.2007.00442.x
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发表时间：
2007-04-01
影响因子：
11.4
通讯作者：
Xiang, Cheng-Bin
中科院分区：
文献类型：
--
作者：
Lei, Zhi-Yong;Zhao, Ping;Xiang, Cheng-Bin
构建了一系列高通量的二克隆载体，为高等植物基因功能分析提供依据。该载体系列包括用于植物表达、启动子分析、基因沉默和用于蛋白质定位的绿色荧光蛋白融合的质粒。这些载体利用噬菌体整合酶/切除酶位点提供了高通量和高效的克隆。此外，在多个克隆位点中加入独特的限制性内切位点，使启动子能够替换。整个矢量序列具有完整的序列信息和详细的注释，并免费分发给科学界用于非商业用途。
A series of high-throughput binary cloning vectors were constructed to facilitate gene function analysis in higher plants. This vector series consists of plasmids designed for plant expression, promoter analysis, gene silencing, and green fluorescent protein fusions for protein localization. These vectors provide for high-throughput and efficient cloning utilizing sites for lambda phage integrase/excisionase. In addition, unique restriction sites are incorporated in a multiple cloning site and enable promoter replacement. The entire vector series are available with complete sequence information and detailed annotations and are freely distributed to the scientific community for non-commercial uses.