The wbpM gene in Pseudomonas aeruginosa serogroup O17 resides on a cryptic copy of the serogroup O11 O antigen gene locus.
The wbpM gene in Pseudomonas aeruginosa serogroup O17 resides on a cryptic copy of the serogroup O11 O antigen gene locus.
复制标题
铜绿假单胞菌 O17 血清群中的 wbpM 基因位于 O11 O 血清群抗原基因座的隐秘副本上。
DOI：
10.1111/j.1574-6968.2000.tb09137.x
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发表时间：
2000
影响因子：
2.1
通讯作者：
Goldberg,JB
中科院分区：
文献类型：
--
作者：
Dean,CR;Goldberg,JB
铜绿假单胞菌O_（11）群菌株PA_（103）O抗原基因位点由11个基因组成，分别命名为wzz、wzx、wbjA、wzy、wbjB-F、wbpL和wbpM。这些基因中的每一个在20 P中的分布。用Southern印迹法分析铜绿假单胞菌国际抗原分型系统（IATS）血清型。如前所述，wbpM存在于所有20个血清群中。其余O 11 O抗原基因（wzy除外）存在于血清群O 17菌株IATSO 17中，尽管O 11和O 17 O抗原在结构上不相关。测序显示IATSO 17中存在一个神秘血清群O 11基因座，被两个拷贝的1.1 kb插入元件中断。将含有来自菌株PA 103的完整O 11 O抗原位点的质粒pLPS 2导入IATSO 17中，导致O 11和O 17 O抗原的产生。对wbpMin IATSO 17的插入失活结果进行了讨论。
ThePseudomonas aeruginosaserogroup O11 strain PA103 O antigen gene locus consists of 11 genes designatedwzz,wzx,wbjA,wzy,wbjB-F,wbpL, andwbpM. The distribution of each of these genes amongst the 20P. aeruginosainternational antigenic typing system (IATS) serogroups was analyzed by Southern blot. As shown previously,wbpMwas present in all 20 serogroups. The remaining O11 O antigen genes, with the exception ofwzy, were present in the serogroup O17 strain IATSO17, despite the structural unrelatedness of the O11 and O17 O antigens. Sequencing revealed the presence of a cryptic serogroup O11 locus in the IATSO17 interrupted by two copies of a 1.1-kb insertion element. Introduction of plasmid pLPS2, containing the complete O11 O antigen locus from strain PA103, into IATSO17 resulted in production of both the O11 and O17 O antigens. The results of insertional inactivation ofwbpMin IATSO17 are discussed.