Previous chronic exogenous glucocorticoid administration in vivo does not affect functional characteristics and cellular lifespan of human skin fibroblasts in vitro
Previous chronic exogenous glucocorticoid administration in vivo does not affect functional characteristics and cellular lifespan of human skin fibroblasts in vitro
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既往体内长期外源性糖皮质激素给药不影响体外人皮肤成纤维细胞的功能特征和细胞寿命
DOI：
10.1111/j.1600-0625.2011.01262.x
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发表时间：
2011
影响因子：
3.6
通讯作者：
D. Kletsas
中科院分区：
文献类型：
--
作者：
H. Pratsinis;A. Dimozi;Konstantinos Pilichos;S. Tsagarakis;A. Yiacoumettis;D. Kletsas
摘要：据报道，过量的糖皮质激素（GC）会导致皮肤萎缩和伤口愈合受损。本研究调查人类皮肤成纤维细胞是否会因长期接触过量的GC而遭受永久性损伤。从接受 GC 治疗一年以上的患者身上获得的成纤维细胞在标准条件下进行体外培养，并研究了涉及皮肤稳态和衰老的关键参数，即胶原蛋白生成、细胞增殖和细胞复制寿命。与正常人皮肤成纤维细胞相比，没有观察到这些功能的统计差异。此外，在正常细胞和患者来源的细胞对超生理皮质醇浓度的敏感性方面没有观察到差异。总之，人皮肤成纤维细胞在体内长时间暴露于高浓度的外源性GC不会对其生理产生持续的不利影响。
Abstract: Excess of glucocorticoids (GCs) has been reported to lead to skin atrophy and impaired wound healing. The present study investigates whether human skin fibroblasts suffer permanent damages due to a long‐term exposure to GC excess. Fibroblasts obtained from patients being under GC treatment for periods over one year were cultured under standard conditions in vitro, and studied regarding pivotal parameters involved in skin homeostasis and aging, i.e. collagen production, cell proliferation, and cellular replicative lifespan. No statistical differences were observed regarding these functions compared to those of normal human skin fibroblasts. Furthermore, no differences between normal and patient‐derived cells were observed regarding their sensitivity to a supra‐physiological cortisol concentration. In conclusion, the prolonged exposure of human skin fibroblasts in vivo to high concentrations of exogenously‐administered GC does not lead to persistent adverse effects on their physiology.