Pleiotypic control by adenosine 3':5'-cyclic monophosphate: a model for growth control in animal cells.
Pleiotypic control by adenosine 3':5'-cyclic monophosphate: a model for growth control in animal cells.
复制标题
腺苷 3:5-环单磷酸的多型控制：动物细胞生长控制的模型。
DOI：
10.1073/pnas.70.5.1432
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发表时间：
1973
影响因子：
11.1
通讯作者：
G. Tomkins
中科院分区：
文献类型：
--
作者：
R. Kram;P. Mamont;G. Tomkins
血清剥夺对与正常成纤维细胞生长相关的几种一般细胞生化过程（“多型反应”）的影响可以通过在血清存在的情况下用前列腺素E处理这些细胞来模拟。N(6)，O(2)'-二丁基腺苷3':5'-环单磷酸和茶碱抑制膜转运过程，对其他多型反应如总蛋白和RNA合成以及蛋白质降解没有太大影响。在血清饥饿期间，细胞内环AMP的数量增加，当添加血清后再次开始生长时，细胞内环AMP的数量恢复到未饥饿细胞的初始浓度。由猿猴病毒40转化的成纤维细胞比未转化的亲本具有更低的环AMP含量。血清剥夺既不增加环AMP含量，也不显著影响转化细胞的多型反应。环己亚胺导致正常成纤维细胞中环AMP含量的降低，与此同时，环己亚胺抑制剂在无血清细胞中刺激尿苷转运和减缓蛋白质降解的能力相一致。
The effects of serum deprivation on several general cellular biochemical processes ("pleiotypic response") related to the growth of normal fibroblasts can be mimicked by treatment of these cells with prostaglandin E(1) in the presence of serum. N(6),O(2)'-Dibutyryl adenosine 3':5'-cyclic monophosphate and theophylline inhibit the membrane transport processes without much effect on other pleiotypic reactions such as overall protein and RNA synthesis and protein degradation. The amount of intracellular cyclic AMP increases during serum starvation and returns to the initial concentration in unstarved cells when growth is initiated again upon addition of serum. Fibroblasts transformed by simian virus 40 have a lower cyclic AMP content than their untransformed parents. Serum deprivation neither increases cyclic AMP content nor significantly affects the pleiotypic reactions in transformed cells. Cycloheximide causes a decrease in cyclic AMP content of normal fibroblasts coincidentally with the ability of this inhibitor to stimulate uridine transport and slow protein degradation in cells deprived of serum.