Influence of cultivar resistance and inoculum density on root hair infection of canola (Brassica napus) by Plasmodiophora brassicae
Influence of cultivar resistance and inoculum density on root hair infection of canola (Brassica napus) by Plasmodiophora brassicae
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DOI：
10.1111/j.1365-3059.2011.02457.x
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发表时间：
2011-10
期刊：
影响因子：
2.7
通讯作者：
S. Hwang;H. Ahmed;Q. Zhou;S. Strelkov;B. Gossen;G. Peng;G. Turnbull
中科院分区：
文献类型：
--
作者：
S. Hwang;H. Ahmed;Q. Zhou;S. Strelkov;B. Gossen;G. Peng;G. Turnbull
品种抗性和接种密度对根肿病的病原菌芸苔根肿病的油菜根毛的初次感染的发病率的影响，通过显微镜进行了评估。根毛感染的发病率在两个抗性和易感品种增加接种密度，但在易感品种高2 - 3倍，根毛感染和接种密度之间的关系也大大加强，更一致的易感品种。感病品种根毛侵染率在播后6 ~ 8d达到高峰，随后下降。在抗性品种中，它在每次研究的14天持续时间内增加。看来，通过显微镜检查易感油菜的诱饵作物中的根毛感染，可以作为一个有用的工具来估计土壤中的P. brassicae接种水平。在一个单独的试验中，接种密度和根肿病的严重程度，植物生长参数和种子产量之间的关系进行了评估，在温室条件下。感病基因型的接种密度与根肿病严重程度呈显著正相关，与株高和种子产量呈显著负相关。此外，一个单一的种植周期的敏感品种贡献了显着更高水平的休眠孢子的土壤中的温室试验比一个周期的抗性品种，通过定量PCR和显微镜分析评估。
The impact of cultivar resistance and inoculum density on the incidence of primary infection of canola root hairs by Plasmodiophora brassicae, the causal agent of clubroot, was assessed by microscopy. The incidence of root hair infection in both a resistant and a susceptible cultivar increased with increasing inoculum density, but was two- to threefold higher in the susceptible cultivar; the relationship between root hair infection and inoculum density was also substantially stronger and more consistent in the susceptible cultivar. In the susceptible cultivar, the root hair infection rate peaked between 6 and 8 days after sowing and then declined. In the resistant cultivar, it increased over the 14-day duration of each study. It appears that examination of root hair infection by microscopy in a bait crop of susceptible canola could serve as a useful tool for estimating P. brassicae inoculum levels in soil. In a separate trial, the relationship between inoculum density and clubroot severity, plant growth parameters, and seed yield was assessed under greenhouse conditions. Inoculum density in the susceptible genotype was strongly and positively correlated with clubroot severity and negatively correlated with plant height and seed yield. In addition, a single cropping cycle of the susceptible cultivar contributed significantly higher levels of resting spores to the soil in a greenhouse test than did a cycle of the resistant cultivar, as assessed by quantitative PCR and microscope analysis.