Structure-guided rational design of red fluorescent proteins: towards designer genetically-encoded fluorophores
Structure-guided rational design of red fluorescent proteins: towards designer genetically-encoded fluorophores
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DOI：
10.1016/j.sbi.2016.12.001
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发表时间：
2017-08-01
影响因子：
6.8
通讯作者：
Chica, Roberto A.
中科院分区：
文献类型：
--
作者：
Eason, Matthew G.;Damry, Adam M.;Chica, Roberto A.
红色荧光蛋白由于具有较长的激发和发射波长，已成为现代生物学研究的重要组成部分。蛋白质工程的努力已经改善了rfp的许多关键性质，使其在成像中的实际应用。即便如此，仍需要持续的工程来克服红色发色团的缺点，并创造出具有与优化后的绿色和黄色发色团相媲美的光物理性质的rfp。在这里，我们重点介绍了最近结构导向的rfp合理设计的例子，以提高亮度、单体化、成熟度和光稳定性，并讨论了针对特定应用的设计rfp的未来工程的可能途径。
Red fluorescent proteins (RFPs) have become an integral part of modern biological research due to their longer excitation and emission wavelengths. Protein engineering efforts have improved many key properties of RFPs for their practical use in imaging. Even so, continued engineering is required to overcome the shortcomings of the red chromophore and create RFPs with photophysical properties rivalling those of their optimized green and yellow counterparts. Here, we highlight recent examples of structure-guided rational design of RFPs to improve brightness, monomerization, maturation, and photostability, and discuss possible pathways for the future engineering of designer RFPs tailored to specific applications.