A Flight Sensory-Motor to Olfactory Processing Circuit in the Moth Manduca sexta.
A Flight Sensory-Motor to Olfactory Processing Circuit in the Moth Manduca sexta.
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DOI：
10.3389/fncir.2016.00005
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发表时间：
2016
影响因子：
3.5
通讯作者：
Dacks AM
中科院分区：
文献类型：
--
作者：
Bradley SP;Chapman PD;Lizbinski KM;Daly KC;Dacks AM
将信息从运动路径投射到感觉路径的神经回路在感觉域之间是常见的。由于运动模式激活，这些回路通常会改变感觉功能；在产生的行为影响感官体验或其处理的情况下尤其如此。然而，这种电路还没有在任何物种中被观察到投射到嗅觉通路上，尽管有很好的特征，主动采样行为会产生递指机械刺激，比如哺乳动物的嗅觉和Manduca sexta飞蛾的拍打翅膀。在这项研究中，我们描述了连接曼都卡飞行感觉运动中心和嗅觉中心的电路。该回路由一对组胺免疫反应（HA-ir）神经元组成，这些神经元从胸椎神经节投射到腹侧天线叶（AL）肾小球的一个子集。此外，在AL中，我们发现m.s sexta组胺B受体（MsHisClB）仅由gaba能和肽能的一组LNs表达，这些LNs广泛地投射到所有嗅肾小球。最后，HA-ir细胞对存在于5期幼虫中；然而，MsHisClB-ir在幼虫触角中心的缺失表明该回路在变态之前是不完整的，重要的是在飞行行为表达之前。尽管该回路的功能后果尚不清楚，但这些结果首次详细描述了将嗅觉系统与驱动飞行行为（包括气味引导飞行）的运动中心连接起来的回路。
Neural circuits projecting information from motor to sensory pathways are common across sensory domains. These circuits typically modify sensory function as a result of motor pattern activation; this is particularly so in cases where the resultant behavior affects the sensory experience or its processing. However, such circuits have not been observed projecting to an olfactory pathway in any species despite well characterized active sampling behaviors that produce reafferent mechanical stimuli, such as sniffing in mammals and wing beating in the moth Manduca sexta. In this study we characterize a circuit that connects a flight sensory-motor center to an olfactory center in Manduca. This circuit consists of a single pair of histamine immunoreactive (HA-ir) neurons that project from the mesothoracic ganglion to innervate a subset of ventral antennal lobe (AL) glomeruli. Furthermore, within the AL we show that the M. sexta histamine B receptor (MsHisClB) is exclusively expressed by a subset of GABAergic and peptidergic LNs, which broadly project to all olfactory glomeruli. Finally, the HA-ir cell pair is present in fifth stage instar larvae; however, the absence of MsHisClB-ir in the larval antennal center indicates that the circuit is incomplete prior to metamorphosis and importantly prior to the expression of flight behavior. Although the functional consequences of this circuit remain unknown, these results provide the first detailed description of a circuit that interconnects an olfactory system with motor centers driving flight behaviors including odor-guided flight.