Diversity and conservation of CELF1 and CELF2 RNA and protein expression patterns during embryonic development.
Diversity and conservation of CELF1 and CELF2 RNA and protein expression patterns during embryonic development.
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DOI：
10.1002/dvdy.23959
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发表时间：
2013-06
影响因子：
2.5
通讯作者：
Ladd, A. N.
中科院分区：
文献类型：
--
作者：
Blech-Hermoni, Y.;Stillwagon, S. J.;Ladd, A. N.
CUG-BP、Elav-like家族成员1（CELF 1）和CELF 2是调节RNA加工的几个阶段的RNA结合蛋白，并且在发育和成体组织中广泛表达。在这项研究中，我们研究了CELF 1和CELF 2转录本和蛋白质在不同组织、发育阶段和生物体中的表达模式。我们发现CELF 1和CELF 2蛋白水平在心脏发育过程中独立于转录水平进行调节，并且这些蛋白在胚胎心脏中表现出核和细胞质同种型。我们发现CELF 1的亚细胞分布在心脏，肝脏，神经系统和眼睛之间存在差异，并在这些组织中鉴定了CELF 1和CELF 2的组织特异性亚型。CELF 1和CELF 2在很大程度上是共表达的，但在几个器官（包括心脏和眼睛）中发现了相互排斥的区域。最后，我们发现，在鸡胚中观察到的表达模式主要是重演在发育中的小鼠，这表明这些蛋白质的组织和细胞的发育胚胎的作用是保守的。CELF 1和CELF 2可能是脊椎动物胚胎中保守的、发育调节的、组织特异性过程的基础。不同的组织可能具有独特的细胞核和细胞质CELF 1和CELF 2介导的功能。
CUG-BP, Elav-like family member 1 (CELF1) and CELF2 are RNA-binding proteins that regulate several stages of RNA processing, and are broadly expressed in developing and adult tissues. In this study, we investigated the expression patterns of CELF1 and CELF2 transcripts and proteins in different tissues, stages of development, and organisms. We found that CELF1 and CELF2 protein levels are regulated independently of transcript levels during heart development, and these proteins exhibit nuclear and cytoplasmic isoforms in the embryonic heart. We found that the subcellular distribution of CELF1 differs between heart, liver, nervous system, and eye, and identified tissue-specific isoforms of both CELF1 and CELF2 in these tissues. CELF1 and CELF2 are largely co-expressed, but are found in mutually exclusive territories in several organs, including the heart and eye. Finally, we show that the expression patterns observed in embryonic chicken were mostly recapitulated in the developing mouse, suggesting that the roles of these proteins in the tissues and cells of the developing embryo are conserved as well. CELF1 and CELF2 may underlie conserved, developmentally regulated, tissue-specific processes in vertebrate embryos. Different tissues likely have unique profiles of nuclear and cytoplasmic CELF1- and CELF2-mediated functions.
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影响因子：
5.3
作者：
Baldwin, BR;Timchenko, NA;Zahnow, CA
通讯作者：
Zahnow, CA
影响因子：
9.8
作者：
Magdaleno S;Jensen P;Brumwell CL;Seal A;Lehman K;Asbury A;Cheung T;Cornelius T;Batten DM;Eden C;Norland SM;Rice DS;Dosooye N;Shakya S;Mehta P;Curran T
通讯作者：
Curran T
影响因子：
4.8
作者：
Ladd, AN;Nguyen, NH;Cooper, TA
通讯作者：
Cooper, TA
DOI：
10.1196/annals.1299.093
发表时间：
2003-01-01
期刊：
APOPTOSIS: FROM SIGNALING PATHWAYS TO THERAPEUTIC TOOLS
影响因子：
--
作者：
Mukhopadhyay, D;Jung, J;Anant, S
通讯作者：
Anant, S
影响因子：
4
作者：
Ladd, AN;Cooper, TA
通讯作者：
Cooper, TA