RIBONUCLEIC-ACID ISOLATED BY CESIUM-CHLORIDE CENTRIFUGATION
RIBONUCLEIC-ACID ISOLATED BY CESIUM-CHLORIDE CENTRIFUGATION
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DOI：
10.1021/bi00709a025
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发表时间：
1974-01-01
期刊：
影响因子：
2.9
通讯作者：
BYUS, C
中科院分区：
文献类型：
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作者：
GLISIN, V;CRKVENJAKOV, R;BYUS, C
弗拉基米尔Glisin，J拉多米尔Crkvenjakov，* 和克雷格Byus §摘要：描述了一种基于CsCl超离心从破碎细胞或亚细胞组分中分离RNA的快速简单方法。获得完整的rRNA，并且可以以接近100%的产率回收。结果表明，兔珠蛋白mRNA的翻译不受这种处理的影响。用此方法提取的珠蛋白mRNA经poly（U）-Sepharose柱部分富集后，在小麦胚S-30提取液和苯酚提取液中测定了含poly（A）珠蛋白mRNA的产量。CsCl提取的RNA在珠蛋白合成中的活性是CsCl提取的RNA的6倍。据观察，特定的聚集发生在
Vladimir Glisin, J Radomir Crkvenjakov,* and Craig Byus § abstract: A quick and simple procedure based upon CsCl ultracentrifugation for the isolation of RNA from disrupted cells or subcellular fractions is described. rRNA is obtained intact and can be recovered in yields close to 100%. It is shown that the translation of rabbit globin mRNA is not impaired byexposure to this treatment. The yield of poly (A) containing globin mRNA extracted by this method and par-tially enriched on a poly (U)-Sepharose column was assayed in wheat germ S-30 extractsalong with that extracted with phenol undergoing identical treatment. RNA from the CsCl extraction yields RNA six times more active in globin synthe-sis. It is observed that specific aggregation takes place during