Characterization of bacterial community structure in vestimentiferan tubeworm Ridgeia piscesae trophosomes
Characterization of bacterial community structure in vestimentiferan tubeworm Ridgeia piscesae trophosomes
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DOI：
10.1111/j.1439-0485.2007.00151.x
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发表时间：
2007-03-01
影响因子：
1.1
通讯作者：
Moyer, Craig L.
中科院分区：
文献类型：
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作者：
Chao, Leslie S. -L.;Davis, Richard E.;Moyer, Craig L.
末端限制性片段长度多态性（T-RFLPs）被用来解决的问题是否有多种细菌的营养体内的vestimentiferan管虫Ridgeia piscesae除了已知的内共生菌。构建克隆文库以帮助鉴定另外的细菌噬菌体类型。个别河鱼类标本采自Juan de Fuca Ridge，Axial Caldera和Explorer Ridge，Magic Mountain。克隆文库分析显示，只有一种细菌性大肠杆菌类型（预期为R。鱼内共生体）内的探险家岭滋养体。然而，Axial Caldera克隆库揭示了五个操作分类单位（OTU）。检测到的三种所得到的细菌类型（指定为RAE）是γ-变形菌门内的假定硫自养共生体，其属于预期的内共生体组（RAE OTU 1）、Maorithyas hadalis共生体II组（RAE OTU 3）和Halothiobacillus组（RAE OTU 4）。剩下的两种病毒很可能是机会主义衍生的。RAE OTU 5是Rosebrum组内的α-变形杆菌，RAE OTU 2是噬细胞-黄杆菌拟杆菌内的。T-RFLP分析显示，评估的所有15个滋养体至少含有RAE OTU 1和4。15个滋养体中的5个含有至少RAE OTU 1、3和4，并且滋养体中的2个含有至少5个RAE OTU。聚类分析的T-RFLP数据显示三个不同的集群。滋养体内的分类群的数量可以指示管虫的总体健康状况。这项研究有力地表明，我们已经发现并确定了一个以上的细菌natriuretic类型内的营养体vestimentiferan R。鱼
Terminal-restriction fragment length polymorphisms (T-RFLPs) were used to address the question whether there are multiple phylotypes of bacteria within the trophosome of the vestimentiferan tubeworm Ridgeia piscesae in addition to the known endosymbiont. Clone libraries were constructed to aid in the identification of the additional bacterial phylotypes. Individual R. piscesae specimens were collected from Juan de Fuca Ridge, Axial Caldera and Explorer Ridge, Magic Mountain. Clone library analyses revealed only one bacterial phylotype (expected R. piscesae endosymbiont) within the Explorer Ridge trophosomes. However, the Axial Caldera clone library revealed five operational taxonomic units (OTUs). Three of the resulting phylotypes detected (designated RAE) were putative thioautotrophic symbionts within the gamma-Proteobacteria which belonged to the anticipated endosymbiont group (RAE OTU 1), the Maorithyas hadalis symbiont II group (RAE OTU 3), and the Halothiobacillus group (RAE OTU 4). The remaining two phylotypes were most likely opportunistically derived. RAE OTU 5 was an a-Proteobacterium within the Roseobacter group and RAE OTU 2 was within the Cytophaga-FlavobacteriumBacteroidetes. T-RFLP analyses revealed that all 15 trophosomes evaluated contained at least RAE OTUs 1 and 4. Five of the 15 trophosomes contained at least RAE OTUs 1, 3, and 4 and two of the trophosomes contained at least five RAE OTUs. Cluster analysis of the T-RFLP data revealed three distinct clusters. The number of taxa within the trophosome may be an indication of the general health of the tubeworm. This study strongly suggests that we have discovered and identified more than one bacterial phylotype within the trophosome of vestimentiferan R. piscesae.