Ebola virus VP40 drives the formation of virus-like filamentous particles along with GP
Ebola virus VP40 drives the formation of virus-like filamentous particles along with GP
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DOI：
10.1128/jvi.76.10.4855-4865.2002
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发表时间：
2002-05-01
影响因子：
5.4
通讯作者：
Kawaoka, Y
中科院分区：
文献类型：
--
作者：
Noda, T;Sagara, H;Kawaoka, Y
使用生物化学测定，已经证明在哺乳动物细胞中单独表达埃博拉病毒VP40诱导产生密度与病毒体相似的颗粒。为了确定这些颗粒的形态学性质，通过电子显微镜检查表达VP 40的细胞和从细胞释放的颗粒。VP40诱导丝状颗粒的质膜出芽，其长度不同，但具有约65 nm的均匀直径。当负责受体结合和膜融合的埃博拉病毒糖蛋白（GP）在细胞中表达时，我们发现多形性颗粒从质膜出芽。与此相反，当GP与VP40共表达时，在VP40诱导的丝状颗粒上发现GP。这些结果表明VP40在埃博拉病毒粒子丝状结构形成中的核心作用，并可能表明VP40和GP在形态发生中的相互作用。
Using biochemical assays, it has been demonstrated that expression of Ebola virus VP40 alone in mammalian cells induced production of particles with a density similar to that of virions. To determine the morphological properties of these particles, cells expressing VP40 and the particles released from the cells were examined by electron microscopy. VP40 induced budding from the plasma membrane of filamentous particles, which differed in length but had uniform diameters of approximately 65 nm. When the Ebola virus glycoprotein (GP) responsible for receptor binding and membrane fusion was expressed in cells, we found pleomorphic particles budding from the plasma membrane. By contrast, when GP was coexpressed with VP40, GP was found on the filamentous particles induced by VP40. These results demonstrated the central role of VP40 in formation of the filamentous structure of Ebola virions and may suggest an interaction between VP40 and GP in morphogenesis.