Chronic constriction injury of the infraorbital nerve in the rat using modified syringe needle
Chronic constriction injury of the infraorbital nerve in the rat using modified syringe needle
复制标题
DOI：
10.1016/j.jneumeth.2008.04.013
复制
发表时间：
2008-07-15
影响因子：
3
通讯作者：
Ohara, Peter T.
中科院分区：
文献类型：
--
作者：
Kernisant, Melanie;Gear, Robert W.;Ohara, Peter T.
在这里，我们报告了一种方法进行慢性压迫性损伤（CCI）的眶下神经（ION）在大鼠作为一个组成部分的慢性疼痛模型。手术方法的离子一起描述了使用一种改进的牙科注射器针头，简化了放置两个铬肠结扎周围的离子。这种方法使外科手术更容易，神经损伤在动物中更一致，并减少对ION和周围组织的二次损伤。疼痛行为测试结合三叉神经脊束核神经损伤标记物的免疫染色显示了该程序作为口面疼痛模型的适用性。(C)2008 Elsevier B.V.保留所有权利。
Here we report a method for performing a chronic constriction injury (CCI) of the infraorbital nerve (ION) in the rat as a component of a chronic pain model. The surgical approach to the ION is described together with the use of a modified dental syringe needle that simplifies placing two chromic gut ligatures around the ION. This method makes the surgical procedure easier, the nerve injury more consistent across animals and reduces secondary damage to the ION and surrounding tissue. Pain behavior testing together with immunostaining for markers of nerve injury in the spinal trigeminal nucleus show the suitability of this procedure as a model of orofacial pain. (C) 2008 Elsevier B.V. All rights reserved.