High resolution pair-distance distribution function P(r) of protein solutions
High resolution pair-distance distribution function P(r) of protein solutions
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DOI：
10.1063/1.3089360
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发表时间：
2009-02-23
影响因子：
4
通讯作者：
Hao, Quan
中科院分区：
文献类型：
--
作者：
Hong, Xinguo;Hao, Quan
通过将精确的小角度和广角 X 射线散射数据组合到 2.15 A 分辨率，从完整的溶液散射曲线中获得了高分辨率的蛋白质分子对距离分布函数 P(r)。间接和直接傅立叶变换在 P(r) 中的 1.4 和 5.1 A 处均表现出两个不同的峰值。 X 射线晶体学数据表明，这些峰对应于两个分子内距离：分别是 C、N 和 O 原子之间的平均键长和 α 螺旋的螺距。因此，可以在溶液中研究蛋白质结构和功能的一些高分辨率方面。
A high resolution pair-distance distribution function P(r) of protein molecules has been obtained from the complete solution scattering curve made by combining accurate small-angle and wide-angle x-ray scattering data out to 2.15 A resolution. Both indirect and direct Fourier transforms exhibit two distinct peaks at 1.4 and 5.1 A in P(r). X-ray crystallographic data demonstrate that these peaks correspond to two intramolecular distances: the average bond length between C, N, and O atoms and the pitch of an alpha-helix, respectively. Hence some high resolution aspects of the structure and function of a protein may be investigated in a solution.