Selective breeding for high and low alcohol preference in mice
Selective breeding for high and low alcohol preference in mice
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DOI：
10.1023/a:1021489922751
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发表时间：
1999-01-01
影响因子：
2.6
通讯作者：
Lumeng, L
中科院分区：
文献类型：
--
作者：
Grahame, NJ;Li, TK;Lumeng, L
高和低酒精偏好（分别为HAP和HAP）小鼠通过10代双向选择两瓶选择酒精消耗的差异。用于选择的祖细胞是HS/lbg小鼠，其是遗传上确定的远系繁殖的原种。在选择期间，小鼠每天24小时可自由饮用10%酒精（v/v）和水，持续30天，并根据整个期间每天消耗的酒精（g/kg）进行选择。可自由进食。在S10，酒精消耗量的线平均值差异很大，HAP小鼠的消耗量超过12 g/kg/天，而HAP小鼠的消耗量低于2 g/kg/天。双向选择的实现遗传力约为0.2。雌性小鼠比雄性小鼠消耗更多的酒精。酒精消除率没有差异，摄入盐或奎宁溶液也没有差异。然而，糖精溶液的消耗量在HAP小鼠中比在HAP小鼠中更大，这与先前发现的甜味偏好和饮酒之间的遗传相关性一致。由于小鼠基因组是相对较好的特点，这些选定的线应该证明是一个有用的工具，用于评估的遗传基础，和表型，与饮酒。
High and low alcohol preference (HAP and LAP, respectively) mice were created by 10 generations of bidirectional selection for differences in two-bottle choice alcohol consumption. The progenitors used for selection were HS/lbg mice, which are a genetically defined, outbred stock. During selection, mice had 24-h, daily access to 10% alcohol (v/v) and water ad libitum for 30 days and were selected based on the alcohol (g/kg) consumed per day over the entire period. Food was available ad libitum. At S10, line means for alcohol consumption differed greatly, with consumption of over 12 g/kg per day in the HAP mice and less than 2 g/kg per day in the LAP mice. Realized heritability for bidirectional selection was approximately 0.2. Female mice consumed more alcohol than male mice. There were no differences between lines in alcohol elimination rate, nor were there line differences in intake of salt or quinine solutions. However, consumption of saccharin solutions was greater in HAP mice than LAP mice, consistent with previous findings of a genetic correlation between sweet preference and alcohol drinking. Because the mouse genome is relatively well characterized, these selected lines should prove a useful tool for assessment of the genetic basis of, and phenotypes that correlate with, alcohol drinking.