CHARACTERIZATION OF INTRAMUSCULAR COLLAGEN IN THE MAMMALIAN LEFT-VENTRICLE
CHARACTERIZATION OF INTRAMUSCULAR COLLAGEN IN THE MAMMALIAN LEFT-VENTRICLE
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DOI：
10.1007/bf01908312
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发表时间：
1982-01-01
影响因子：
9.5
通讯作者：
MEDUGORAC, I
中科院分区：
文献类型：
--
作者：
MEDUGORAC, I
Left ventricular collagen of various mammals (cat, cow, dog, pig and rat) was successively extracted with neutral salt, dilute acid solutions and limited pepsin digestion. The distribution of the various types of collagen molecules in pepsin-solubilized ventricular collagen was analyzed electrophoretically on SDS[sodium dodecyl sulfate]-polyacrylamide gels in the presence of 3.6 M urea. Yields of dilute-acid-soluble collagen were only 0.4-0.6% of the total ventricular collagen and < 0.1% with neutral salt solution. Of the total collagen .apprx. 55-90% was extracted by limited pepsin digestion. Disc patterns of pepsin-soluble collagen revealed the presence of dimeric and trimeric components, as well as higher MW aggregates in all samples of nonreduced and reduced ventricular collagen. These findings suggest the presence of an extensive interchain and intermolecular cross-linking network in left ventricular collagen. Comparison of electrophoretic disc gel patterns of reduced and nonreduced pepsin-solubilized collagen indicated that left ventricular collagen is heterogenous in nature, consisting of a mixture of type I and type III collagen. Apparently, primarily type I components occur in ventricular collagen. The components of type III collagen molecules occurred in all investigated left ventricles in varying and consistently lower amounts. The proportions of type III and type I collagen vary in left ventricular tissue of different species.
