Transcriptomic analysis reveals the roles of microtubule-related genes and transcription factors in fruit length regulation in cucumber (Cucumis sativus L.)
Transcriptomic analysis reveals the roles of microtubule-related genes and transcription factors in fruit length regulation in cucumber (Cucumis sativus L.)
复制标题
转录组分析揭示了微管相关基因和转录因子在黄瓜 (Cucumis sativus L.) 果实长度调节中的作用
DOI：
10.1038/srep08031
复制
发表时间：
2015-01-26
影响因子：
4.6
通讯作者：
Zhang, Xiaolan
中科院分区：
文献类型：
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作者：
Jiang, Li;Yan, Shuangshuang;Zhang, Xiaolan
黄瓜（Cucumis sativus L.）水果是一种立即收获的肉质水果。果实的早期发育直接决定着最终果实的长度和直径，进而决定着果实的产量和品质。不同的黄瓜品种表现出巨大的果实长度的变化，但果实长度是如何在分子水平上确定仍然知之甚少。为了了解调控黄瓜果实长度的基因和基因网络，使用高通量RNA-Seq数据来比较来自具有不同果实长度的两个近等基因系的早期果实的转录组。共检测到3955个差异表达基因，其中2368个基因在长果系中表达显著上调，1587个基因在长果系中表达显著下调。微管和细胞周期相关基因在长果中被显着激活，转录因子参与了黄瓜果实长度的调节。因此，我们的研究结果奠定了基础，为剖析黄瓜，一个重要的经济意义的农业性状的果实长度控制的分子机制。
Cucumber (Cucumis sativus L.) fruit is a type of fleshy fruit that is harvested immaturely. Early fruit development directly determines the final fruit length and diameter, and consequently the fruit yield and quality. Different cucumber varieties display huge variations of fruit length, but how fruit length is determined at the molecular level remains poorly understood. To understand the genes and gene networks that regulate fruit length in cucumber, high throughout RNA-Seq data were used to compare the transcriptomes of early fruit from two near isogenic lines with different fruit lengths. 3955 genes were found to be differentially expressed, among which 2368 genes were significantly up-regulated and 1587 down-regulated in the line with long fruit. Microtubule and cell cycle related genes were dramatically activated in the long fruit, and transcription factors were implicated in the fruit length regulation in cucumber. Thus, our results built a foundation for dissecting the molecular mechanism of fruit length control in cucumber, a key agricultural trait of significant economic importance.