Serum microRNA-125b correlates with hepatitis B viral replication and liver necroinflammation
Serum microRNA-125b correlates with hepatitis B viral replication and liver necroinflammation
复制标题
血清 microRNA-125b 与乙型肝炎病毒复制和肝脏坏死性炎症相关。
DOI：
10.1016/j.cmi.2015.12.024
复制
发表时间：
2016-04-01
影响因子：
14.2
通讯作者：
Zhang, J.
中科院分区：
文献类型：
--
作者：
Li, F.;Zhou, P.;Zhang, J.
最近报道 MicroRNA (miRNA) 在乙型肝炎病毒 (HBV) 感染及相关疾病中发挥重要作用。我们评估了中国慢性乙型肝炎 (CHB) 感染患者的血清 miRNA-125b、病毒复制和肝脏坏死性炎症之间的相关性。通过 RT-PCR 测定了 211 名 CHB 患者样本中的血清 miRNA-125b 水平。从 138 名患者身上采集了肝活检。测定血清 miRNA-125b、miRNA-122 和 miRNA-124 水平。分析了血清 miRNA-125b、病毒复制和肝脏坏死性炎症之间的相关性。使用受试者工作特征 (ROC) 曲线评估血清 miRNA-125b 对肝脏坏死性炎症 (G) 分级的区分能力。 HepG2.2.15 细胞用 miRNA-125b 模拟物转染。提取细胞内病毒核心DNA并通过Southern印迹进行分析。我们发现血清miRNA-125b与血清HBV DNA水平呈正相关。转染 miRNA-125b 模拟物后，HBV 复制能力增加。患有中重度肝脏坏死性炎症 (G >= 2) 的 CHB 患者的血清 miRNA-125b 水平显着高于 G < 2 的患者 (p < 0.001)。在丙氨酸转氨酶水平低于正常上限两倍的患者中，血清 miRNA-125b 与 miRNA-124 结合得出的 ROC 曲线下面积为 0.816，灵敏度为 70.4%区分肝脏坏死性炎症级别的特异性为 84.9% (G >= 2)。因此，我们得出结论，miRNA-125b 可能增强 HBV 复制。血清 miRNA-125b 与病毒载量相关。单独的血清 miRNA-125b 或与 miRNA-124 联合使用有可能区分肝脏坏死性炎症的分级，特别是对于丙氨酸转氨酶水平正常或轻度升高的中国慢性乙型肝炎患者。 (C) 2016 年欧洲临床微生物学和传染病学会。由爱思唯尔有限公司出版。保留所有权利。
MicroRNAs (miRNAs) were recently reported to play an important role in hepatitis B virus (HBV) infection and related diseases. We evaluated the correlation between serum miRNA-125b, viral replication and liver necroinflammation in Chinese patients with chronic hepatitis B (CHB) infection. Serum miRNA-125b levels in samples from 211 CHB patients were determined by RT-PCR. Liver biopsies were collected from 138 patients. Serum miRNA-125b, miRNA-122 and miRNA-124 levels were determined. Correlations between serum miRNA-125b, viral replication and liver necroinflammation were analysed. The receiver operating characteristic (ROC) curve was used to assess the discriminating power of serum miRNA-125b to grade liver necroinflammation (G). HepG2.2.15 cells were transfected with miRNA-125b mimics. Intracellular viral core DNA was extracted and analysed by Southern blot. We found that serum miRNA-125b was positively correlated with the serum HBV DNA level. HBV replication capacity increased after transfection with miRNA-125b mimics. Patients with CHB with moderate-to-severe liver necroinflammation (G >= 2) showed significantly higher (p < 0.001) serum miRNA-125b levels than those with G < 2. In patients with alanine transaminase levels less than twice the upper limit of normal, serum miRNA-125b combined with miRNA-124 yielded an area under the ROC curve of 0.816, with 70.4% sensitivity and 84.9% specificity to discriminate the grade of liver necroinflammation (G >= 2). Hence, we concluded that miRNA-125b may enhance HBV replication. Serum miRNA-125b correlates with viral load. Serum miRNA-125b alone or combined with miRNA-124 has the potential to discriminate grades of liver necroinflammation, particularly in Chinese patients with CHB who have normal or mildly increased alanine transaminase levels. (C) 2016 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.