Hepatitis C virus-specific cytolytic T lymphocyte and T helper cell responses in seronegative persons
Hepatitis C virus-specific cytolytic T lymphocyte and T helper cell responses in seronegative persons
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DOI：
10.1086/516546
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发表时间：
1997-10-01
影响因子：
6.4
通讯作者：
Walker, BD
中科院分区：
文献类型：
--
作者：
Koziel, MJ;Wong, DKH;Walker, BD
丙型肝炎病毒（HCV）是一种常见的全球性感染，在大多数人中，它会导致持续的病毒血症和肝损伤。在受感染的人类和唯一的动物模型黑猩猩中，这种高持续感染率阻碍了确定保护性免疫相关性的努力。来自血清阴性者的外周血单个核细胞用代表自然感染后HCV特异性细胞毒性T淋巴细胞（CTL）识别的表位的合成肽刺激。此外，在这些相同的人中检测了对重组HCV蛋白的CD4（+）增殖反应。在2例HCV阴性的职业暴露者中发现了针对HCV抗原表位的CTL反应和增殖反应，这些健康人没有病毒血症，提示他们可能已从急性HCV感染中恢复。暴露但血清阴性者的病毒特异性免疫应答特征可能为HCV保护性免疫的性质提供重要线索。
Hepatitis C virus (HCV) is a common infection worldwide, and in most persons, it leads to persistent viremia and liver damage. Efforts to identify the correlates of protective immunity are hampered by this high rate of persistent infection in both infected humans and the only animal model, the chimpanzee. Peripheral blood mononuclear cells from seronegative persons were stimulated with synthetic peptides that represent epitopes recognized by HCV-specific cytotoxic T lymphocytes (CTL) after natural infection. In addition, CD4(+) proliferative responses to recombinant HCV proteins were examined in these same persons. CTL responses directed against a peptide epitope of HCV and proliferative responses in 2 HCV-seronegative persons with possible occupational exposure to HCV were found. These otherwise healthy persons were not viremic, suggesting that they may have recovered from acute HCV infection. Characterization of virus-specific immune responses in exposed but seronegative persons may provide important clues as to the nature of protective immunity in HCV.