Vascular endothelial growth factor (VEGF) and angiopoietin regulation by gonadotrophin and steroids in macaque granulosa cells during the peri-ovulatory interval
Vascular endothelial growth factor (VEGF) and angiopoietin regulation by gonadotrophin and steroids in macaque granulosa cells during the peri-ovulatory interval
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DOI：
10.1093/molehr/5.12.1115
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发表时间：
1999-12-01
影响因子：
4
通讯作者：
Stouffer, RL
中科院分区：
文献类型：
--
作者：
Hazzard, TM;Molskness, TA;Stouffer, RL
实验首先检测颗粒细胞中血管内皮生长因子（VEGF）、血管生成素-1 （ang1）和血管生成素-2 （ang2）的表达，其次确定促性腺激素和/或类固醇激素是否在排卵期调节其表达。在注射人绒毛膜促性腺激素（HCG）后（0小时）、12小时、36小时、类固醇消融和黄体酮替代或不使用)进行卵巢刺激的恒河猴身上采集颗粒细胞和卵泡液。VEGF;在刺激排卵前均检测到Ang-1和Ang-2 mRNA。虽然卵泡液VEGF浓度在0 ~ 12 h期间增加了6倍（P < 0.05），但VEGF mRNA值不变，不受类固醇消融术的影响。从0到12 h， Ang-1 mRNA下降（P < 0.05）， 36 h， Ang-2 mRNA增加30倍（P < 0.05），而在0、12和36 h之间，Ang-1 mRNA的值不变。类固醇消融在36 h和12 h降低Ang-1 mRNA (P < 0.05)，而黄体酮替代仅使Ang-1 mRNA恢复。这些数据表明，血管特异性生长因子在灵长类动物排卵泡颗粒细胞中以促性腺激素依赖性、类固醇依赖性（VEGF）或类固醇依赖性（Ang-1）的方式动态表达。
The role of endothelial cell-specific growth factors in the vascularization of the primate peri-ovulatory follicle was examined, Experiments were designed firstly to detect expression of vascular endothelial growth factor (VEGF), angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2) in granulosa cells and secondly, to determine whether gonadotrophins and/or steroids regulate their expression during the peri-ovulatory interval. Granulosa cells and follicular fluid were collected from rhesus macaques undergoing ovarian stimulation before (0 h), 12, or 36 h after a bolus of ovulatory human chorionic gonadotrophin (HCG), with or without steroid ablation and progestin replacement. VEGF; Ang-1 and Ang-2 mRNA were all detected prior to the ovulatory stimulus. Whereas follicular fluid VEGF concentrations increased 6-fold (P < 0.05) between 0 and 12 h, VEGF mRNA values were unchanged and were unaffected by steroid ablation. Ang-l mRNA decreased from 0 to 12 h (P < 0.05), followed by a 30-fold increase (P < 0.05) at 36 h, while Ang-2 mRNA values were unchanged between 0, 12 and 36 h. Steroid ablation decreased (P < 0.05) Ang-1 mRNA at 36 h, and Ang-2 mRNA at 12 h, while only Ang-1 was restored by progestin replacement. These data suggest a dynamic expression of vascular-specific growth factors in a gonadotrophin-dependent, steroid-independent (VEGF) or steroid-dependent (Ang-1) manner in granulosa cells of peri-ovulatory follicles of primates.