Gene expression of WWOX, FHIT and p73 in acute lymphoblastic leukemia.
Gene expression of WWOX, FHIT and p73 in acute lymphoblastic leukemia.
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DOI：
10.3892/ol.2013.1514
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发表时间：
2013-10
期刊：
影响因子：
2.9
通讯作者：
Mo W
中科院分区：
文献类型：
--
作者：
Chen X;Zhang H;Li P;Yang Z;Qin L;Mo W
本研究的目的是分析急性淋巴细胞白血病（ALL）中WWOX、FHIT和p73的表达。本研究采集了122例ALL患者和35例非ALL对照患者的样本。RT-PCR检测WWOX、FHIT和p73 mRNA的表达。采用甲基化特异性PCR方法分析WWOX启动子区、FHIT启动子区和p73第一外显子区的甲基化状态。ALL组WWOX、FHIT和p73的mRNA表达均显著低于对照组（分别为48.2%、42.9%和55.4%）。相比之下，WWOX、FHIT和p73的甲基化频率在ALL样品中显著高于对照组（分别为44.6%、46.4%和37.5%）。这三个基因的mRNA表达与ALL样本中的甲基化频率呈负相关（WWOX、FHIT和p73的相关系数分别为-0.661、-0.685和-0.536）。WWOX与FHIT和p73的mRNA表达呈正相关（相关系数分别为0.569和0.556）。然而，WWOX的甲基化状态与FHIT或p73的甲基化状态无关。提示WWOX、FHIT和p73基因的高甲基化状态可能导致这些基因的表达失活和沉默，促进ALL的发生和发展。确定WWOX、FHIT和p73的mRNA表达和甲基化状态可能有助于ALL治疗方法的发展。
The aim of the present study was to analyze the expression of WW-domain oxidoreductase (WWOX), fragile histidine triad (FHIT) and p73 in acute lymphoblastic leukemia (ALL). Samples from 122 ALL patients and 35 non-ALL control patients were collected in this study. RT-PCR was performed to detect the mRNA expression of WWOX, FHIT and p73. The methylation status of the WWOX promoter region, FHIT promoter region and the first exon region of p73 were also analyzed using the methylation-specific PCR method. The mRNA expression of WWOX, FHIT and p73 was significantly lower in the ALL samples compared with the controls (48.2, 42.9 and 55.4%, respectively). By contrast, the methylation frequency of WWOX, FHIT and p73 was significantly higher in the ALL samples compared with the controls (44.6, 46.4 and 37.5%, respectively). The mRNA expression of these three genes was inversely correlated with the methylation frequency in the ALL samples (correlation coefficients, −0.661, −0.685 and −0.536 for WWOX, FHIT and p73, respectively). Moreover, the mRNA expression of WWOX was positively correlated with that of FHIT and p73 (correlation coefficients, 0.569 and 0.556, respectively). However, the methylation status of WWOX had no correlation with that of FHIT or p73. It was concluded that the high methylation status of WWOX, FHIT and p73 may lead to the inactivation of expression and the silencing of these genes, promoting the occurrence and development of ALL. The determination of the mRNA expression and methylation status of WWOX, FHIT and p73 may aid in the development of treatment approaches for ALL.
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DOI：
10.1073/pnas.93.18.9821
发表时间：
1996-09-03
影响因子：
11.1
作者：
Herman, JG;Graff, JR;Baylin, SB
通讯作者：
Baylin, SB
影响因子：
3.5
作者：
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通讯作者：
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DOI：
10.1073/pnas.0400805101
发表时间：
2004-03-30
影响因子：
11.1
作者：
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通讯作者：
Croce, CM
影响因子：
11.5
作者：
Donati, Valentina;Fontanini, Gabriella;Aqeilan, Rami Ishaq
通讯作者：
Aqeilan, Rami Ishaq
影响因子：
11.4
作者：
Iwai, M;Kiyoi, H;Naoe, T
通讯作者：
Naoe, T