Rapid Identification of Major Histocompatibility Complex Class I Haplotypes Using Deep Sequencing in an Endangered Old World Monkey.
Rapid Identification of Major Histocompatibility Complex Class I Haplotypes Using Deep Sequencing in an Endangered Old World Monkey.
复制标题
利用深度测序快速鉴定濒临灭绝的旧世界猴的主要组织相容性复合体 I 类单倍型。
DOI：
10.1007/s12686-016-0516-0
复制
发表时间：
2016
影响因子：
1.1
通讯作者：
Ting,Nelson
中科院分区：
文献类型：
--
作者：
Simons,NoahD;Ruiz-Lopez,MariaJose;Chapman,ColinA;Goldberg,TonyL;Karl,JulieA;Wiseman,RogerW;Bohn,PatrickS;O'Connor,DavidH;Ting,Nelson
Immunogenetic data from wild primate populations have been difficult to obtain, due to logistic and methodological constraints. We applied a well-characterized deep sequencing method for MHC I typing, developed for macaques, to a population of wild red colobus to assess the feasibility of identifying MHC I-A/B haplotypes. Ten individuals produced sufficient data from blood and tissue samples to assign haplotypes. Eighty-two sequences were classified as red colobus MHC I alleles distributed across six MHC I loci. Individuals averaged ~13 k reads across six MHC I loci, with 83 % of all alleles representing either MHC I-A or MHC I-B loci. This study not only represents an important advance in the identification and genotyping of MHC in the endangered red colobus but also shows the potential for using this approach in other endangered wild primates.
