Involvement of interferon regulatory factor 3 from the barbel chub Squaliobarbus curriculus in the immune response against grass carp reovirus.
Involvement of interferon regulatory factor 3 from the barbel chub Squaliobarbus curriculus in the immune response against grass carp reovirus.
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DOI：
10.1016/j.gene.2018.01.048
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发表时间：
2018-03
期刊：
影响因子：
3.5
通讯作者：
Ronghua Wang;Yaoguo Li;Zhiyu Zhou;Qiaolin Liu;Lingbing Zeng;T. Xiao
中科院分区：
文献类型：
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作者：
Ronghua Wang;Yaoguo Li;Zhiyu Zhou;Qiaolin Liu;Lingbing Zeng;T. Xiao
赤眼鳟是我国重要的经济鱼类，对草鱼呼肠孤病毒（GCRV）具有较强的抗性。本研究克隆了赤眼鳟干扰素调节因子3（Interferon Regulatory Factor 3，IRF 3）的cDNA序列，命名为ScIRF 3，并研究了其抗GCRV的作用。ScIRF 3的cDNA全长1837 bp，包含一个1374 bp的开放阅读框，编码457个氨基酸残基。ScIRF 3蛋白含有保守的结构域，包括N端DNA结合结构域、C端IRF结合结构域和富含丝氨酸的结构域。系统进化分析表明，ScIRF 3与鲫鱼和草鱼IRF 3的亲缘关系较近。实时荧光定量聚合酶链反应分析表明，ScIRF3在赤眼鳟脾脏中的表达量最高，肌肉中的表达量最低。GCRV感染后，ScIRF3和I型干扰素（IFN）在脾脏和肠道的表达水平先上调后下调。相关分析表明，肠组织中IIFN和ScIRF3的表达水平呈显著正相关（Pearson相关系数：0.883，P：0.004）。在GCRV感染的过表达ScIRF3的草鱼肾细胞中，IIFN的表达水平显著上调，而GCRV的滴度显著降低（P<0.05）。这些结果表明ScIRF 3可能在赤眼鳟抗GCRV的I型IFN免疫应答中起作用，并能抑制GCRV在草鱼肾细胞中的复制。
The barbel chubSqualiobarbus curriculusis an important commercial fish species in China, and has shown significant resistance to grass carp reovirus (GCRV). In this study, the cDNA sequence of interferon regulatory factors 3 (IRF3) fromSqualiobarbus curriculus, designated asScIRF3, was cloned, and its effect against GCRV was investigated. The full-length 1837 base pair (bp) cDNA ofScIRF3contained a complete open reading frame of 1374 bp and encoded a putative polypeptide of 457 amino acid residues. TheScIRF3protein contained conserved domains, including an N-terminal DNA-binding domain, a C-terminal IRF association domain, and a serine-rich domain. Phylogenetic analysis showed thatScIRF3was closely clustered withIRF3sfromCarassius auratusandCtenopharyngodon idellus. Quantitative real-time polymerase chain reaction analysis showed that the expression levels ofScIRF3inSqualiobarbus curriculuswere the highest in the spleen and lowest in the muscle. After GCRV infection, expression levels of bothScIRF3and type I interferon (IFN) were initially up-regulated and subsequently down-regulated in the spleen and intestine. Correlation analysis showed that the expression level of type IIFNis significantly positively correlated with that ofScIRF3(Pearson correlation coefficient: 0.883,P: 0.004) in the intestine. The expression level of type IIFNwas also significantly up-regulated and the GCRV titer was significantly decreased (P< .05) in GCRV-infectedScIRF3-overexpressingCtenopharyngodon idelluskidney cells. These results indicate thatScIRF3may play a role in the type IIFNimmune response against GCRV inSqualiobarbus curriculusand can also inhibit GCRV replication inCtenopharyngodon idelluskidney cells.