Endophytic bacterial diversity in roots of Phragmites australis in constructed Beijing Cuihu Wetland (China)
Endophytic bacterial diversity in roots of Phragmites australis in constructed Beijing Cuihu Wetland (China)
复制标题
DOI：
10.1111/j.1574-6968.2010.02015.x
复制
发表时间：
2010-08-01
影响因子：
2.1
通讯作者：
Zhang, Qiang
中科院分区：
文献类型：
--
作者：
Li, Yan Hong;Zhu, Jing Nan;Zhang, Qiang
利用16SRRNA文库技术对北京翠湖湿地中国芦苇根部内生细菌的群落结构和多样性进行了研究。利用引物799f和1492r成功地扩增出细菌16S rRNA片段，并构建了克隆文库。通过菌落聚合酶链式反应对166个个体序列进行验证，对芦根内生细菌的多样性进行了评价。系统发育分析表明，78.9%的克隆隶属于变形杆菌，包括所有5个纲。其他克隆分别为Firmicuts(9.0%)、Cytophaga/Flexibacter/Bacterids(6.6%)、梭杆菌(2.4%)，其中近3.0%为未鉴定细菌。变形杆菌中，Alpha和Gamma亚群最为丰富，分别占变形杆菌总数的34.4%和31.3%，优势属为多形单胞菌、固氮螺杆菌和气单胞菌。此外，近13.6%的变形杆菌与硫酸盐还原细菌(SRB)的某些属非常相似，如十氯单胞菌、硫化弧菌和硫酸盐螺旋菌。这些属的细菌被认为在湿地系统中氮、磷、硫和一些有机化合物的代谢中起着重要作用。因此，这项研究表明，在芦根中发现的各种细菌群落中，内生菌株可能具有很强的潜力来加强芦苇湿地对植物的修复。
The community structure and diversity of endophytic bacteria in reed (Phragmites australis) roots growing in the Beijing Cuihu Wetland, China was investigated using the 16S rRNA library technique. Primers 799f and 1492r were used to amplify the specific bacterial 16S rRNA fragments successfully and construct the clone library. In total, 166 individual sequences were verified by colony PCR and used to assess the diversity of endophytic bacteria in reed roots. Phylogenetic analysis revealed that 78.9% of the clones were affiliated with Proteobacteria and included all five classes. Other clones belonged to Firmicutes (9.0%), Cytophaga/Flexibacter/Bacteroids (6.6%), Fusobacteria (2.4%), and nearly 3.0% were unidentified bacteria. In Proteobacteria, the Alpha and Gamma subgroups were the most abundant, accounting for approximately 34.4% and 31.3% of all Proteobacteria, respectively, and the dominant genera included Pleomorphomonas, Azospirillum, and Aeromonas. In addition, nearly 13.6% of the Proteobacteria were very similar to some genera of sulfate-reducing bacteria (SRB) such as Dechloromonas, Desulfovibrio, and Sulfurospirillum. The bacteria in these genera are considered to play important roles in the metabolism of nitrogen, phosphorus, sulfur, and some organic compounds in wetland systems. Hence, this study demonstrates that within the diverse bacterial communities found in reed roots, endophytic strains might have a strong potential to enhance phytoremediation by reed wetlands.