A brain circuit that synchronizes growth and maturation revealed through Dilp8 binding to Lgr3
A brain circuit that synchronizes growth and maturation revealed through Dilp8 binding to Lgr3
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DOI：
10.1126/science.aac6767
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发表时间：
2015-11-13
期刊：
影响因子：
56.9
通讯作者：
Dominguez, Maria
中科院分区：
文献类型：
--
作者：
Vallejo, Diana M.;Juarez-Carreno, Sergio;Dominguez, Maria
身体尺寸的恒定性和对称性是发育稳定性的标志。然而，目前尚不清楚发育中的动物究竟如何缓冲体型变化。果蝇胰岛素样肽 Dilp8 对生长扰动敏感并控制稳态机制，协调调节生长和成熟以将大小维持在正常范围内。在此我们表明 Lgr3 是 Dilp8 受体。通过使用功能性和腺苷 3',5'-单磷酸测定，我们定义了一对介导稳态调节的 Lgr3 神经元。这些神经元具有广泛的轴突分枝，突触伙伴之间的遗传和绿色荧光蛋白重建表明，这些神经元与胰岛素生成细胞和促前胸腺激素生成神经元连接，以减弱生长和成熟。这种以前未被识别的电路表明，生长和成熟速率根据 Dilp8 信号进行匹配和共同调节，以稳定生物体大小。
Body-size constancy and symmetry are signs of developmental stability. Yet, it is unclearexactly how developing animals buffer size variation. Drosophila insulin-like peptide Dilp8is responsive to growth perturbations and controls homeostatic mechanisms thatcoordinately adjust growth and maturation to maintain size within the normal range. Herewe show that Lgr3 is a Dilp8 receptor. Through the use of functional and adenosine3',5'-monophosphate assays, we defined a pair of Lgr3 neurons that mediate homeostaticregulation. These neurons have extensive axonal arborizations, and genetic and greenfluorescent protein reconstitution across synaptic partners show that these neuronsconnect with the insulin-producing cells and prothoracicotropic hormone-producingneurons to attenuate growth and maturation. This previously unrecognized circuit suggestshow growth and maturation rate are matched and co-regulated according to Dilp8 signalsto stabilize organismal size.