Evidence for two different types of insecticidal P2 toxins with dual specificity in Bacillus thuringiensis subspecies
Evidence for two different types of insecticidal P2 toxins with dual specificity in Bacillus thuringiensis subspecies
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苏云金芽孢杆菌亚种中两种不同类型的具有双重特异性的杀虫 P2 毒素的证据
DOI：
10.1128/jb.171.9.5141-5147.1989
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发表时间：
1989
影响因子：
3.2
通讯作者：
D. Ellar
中科院分区：
文献类型：
--
作者：
C. N. Nicholls;Wasim Ahmad;D. Ellar
来自不同苏云金芽孢杆菌菌株的结晶δ-内毒素中的多肽的分析揭示了两种抗原相似形式的P2蛋白，其在分子量、肽谱和氨基酸序列上不同。这两种形式的纯化制剂显示出P2蛋白在体内对鳞翅目和双翅目成员的特征性双重毒性，但对测试的昆虫的效力明显不同。这两个物种的P2原毒素，溶解和激活的顺序蛋白水解与昆虫肠提取物和α-糜蛋白酶，保留在体内和体外的活性，尽管从N末端去除144个残基。对于低分子量形式，双重杀虫活性在体外测定中是可再现的。
Analysis of polypeptides in the crystalline delta-endotoxins from different Bacillus thuringiensis strains revealed two antigenically similar forms of the P2 protein which differed in molecular mass, peptide profile, and amino acid sequence. Purified preparations of the two forms displayed the characteristic dual toxicity of the P2 protein towards members of the orders Lepidoptera and Diptera in vivo but differed markedly in potency for the insects tested. Both species of the P2 protoxin, solubilized and activated by sequential proteolysis with insect gut extract and alpha-chymotrypsin, retained activity in vivo and in vitro, despite the removal of 144 residues from the N terminus. For the low-molecular-mass form, the dual insecticidal activity was reproducible in the in vitro assays.
DOI：
10.1016/s0021-9258(18)61070-1
发表时间：
1987-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Matsudaira
通讯作者：
P. Matsudaira