Transcriptome analysis of Botrytis cinerea in response to tea tree oil and its two characteristic components
Transcriptome analysis of Botrytis cinerea in response to tea tree oil and its two characteristic components
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DOI：
10.1007/s00253-020-10382-9
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发表时间：
2020-01
影响因子：
5
通讯作者：
Zhenbiao Li;X. Shao;Yingying Wei;Kun Dai;Jiayu Xu;Feng Xu;Hongfei Wang
中科院分区：
文献类型：
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作者：
Zhenbiao Li;X. Shao;Yingying Wei;Kun Dai;Jiayu Xu;Feng Xu;Hongfei Wang
茶树油 (TTO) 及其两种特征成分（terpinen-4-ol 和 1,8-cineole）已被证明可以抑制灰葡萄孢的生长。在这项研究中，我们进行了转录组分析，以确定 TTO 及其特征成分（单独或组合）对抗灰葡萄孢菌的效果。用terpinen-4-ol处理的B.cinerea细胞的大多数差异表达基因（DEG）参与次级代谢产物的生物合成以及氨基酸、碳水化合物和脂质的代谢。所有含有 terpinen-4-ol 的治疗都可能诱发线粒体功能障碍和氧化应激。几种酶（例如琥珀酸脱氢酶（SDH）、苹果酸脱氢酶（MDH）、α-酮戊二酸脱氢酶（α-KGDH）、异柠檬酸脱氢酶（ICDH））活性降低，某些酶（例如过氧化氢酶（CAT）、过氧化物酶（POD）、 超氧化物歧化酶（SOD）），并增加过氧化氢（H2O2）的含量。 1,8-桉叶素主要影响参与遗传信息处理的DEG，导致细胞死亡。本研究深入探讨了TTO抑制B.cinerea的分子机制，并解释了terpinen-4-ol和1,8-cineole对B.cinerea的协同作用。
Tea tree oil (TTO) and its two characteristic components (terpinen-4-ol and 1,8-cineole) have been shown to inhibitBotrytis cinereagrowth. In this study, we conducted a transcriptome analysis to determine the effects of TTO and its characteristic components, alone and in combination, againstB.cinerea. Most differentially expressed genes (DEGs) fromB.cinereacells treated with terpinen-4-ol participated in the biosynthesis of secondary metabolites, and the metabolism of amino acids, carbohydrates, and lipids. All treatments containing terpinen-4-ol potentially induced mitochondrial dysfunction and oxidative stress. These were further confirmed by the decreased activities of several enzymes (e.g., succinate dehydrogenase (SDH), malate dehydrogenase (MDH), α-ketoglutarate dehydrogenase (α-KGDH), isocitrate dehydrogenase (ICDH)), the increased activities of certain enzymes (e.g., catalase (CAT), peroxidase (POD), superoxide dismutase (SOD)), and increased content of hydrogen peroxide (H2O2). 1,8-Cineole mainly affected DEGs involved in genetic information processing, resulting in cell death. This study provides insight into the molecular mechanism ofB.cinereainhibition by TTO, and explains the synergistic effect of terpinen-4-ol and 1,8-cineole onB.cinerea.