Inhibition of influenza virus sialidase and anti-influenza virus activity by plant flavonoids.
Inhibition of influenza virus sialidase and anti-influenza virus activity by plant flavonoids.
复制标题
DOI：
10.1248/cpb.38.1329
复制
发表时间：
1990-05
影响因子：
1.7
通讯作者：
T. Nagai;Y. Miyaichi;T. Tomimori;Y. Suzuki;H. Yamada
中科院分区：
文献类型：
--
作者：
T. Nagai;Y. Miyaichi;T. Tomimori;Y. Suzuki;H. Yamada
使用对硝基苯基-N-乙酰基-α-D-神经氨酸钠作为底物，测试黄酮类化合物（103种）对流感病毒唾液酸酶的抑制活性。来自黄芩根的5，7，4 '-三羟基-8-甲氧基黄酮显示出最强的活性（IC 50，55 μ M），并且该黄酮似乎是酶的非竞争性抑制剂。然而，如所测试的，观察到对小鼠肝唾液酸酶、β-半乳糖苷酶和α-甘露糖苷酶的可忽略不计的或弱的抑制活性。该黄酮还能抑制流感病毒A/PR/8/34对犬肾细胞的感染和病毒在鸡胚尿囊中的复制。这些结果表明，黄酮，这是一个强大的流感病毒唾液酸酶抑制活性，可能具有抗流感病毒活性。
Flavonoids (103 species) were tested for inhibitory activity against influenza virus sialidase using sodium p-nitrophenyl-N-acetyl-alpha-D-neuraminate as substrate. 5,7,4'-Trihydroxy-8-methoxyflavone from the root of Scutellaria baicalensis showed the most potent activity (IC50, 55 microM), and this flavone appeared to be a non-competitive inhibitor of the enzyme. Whereas, negligible or weak inhibitory activities were observed for mouse liver sialidase, beta-galactosidase and alpha-mannosidase as tested. This flavone also inhibited the infection by influenza virus A/PR/8/34 of Madin-Darby canine kidney cells, and replication of the virus in the allantoic sack of embryonated egg. These results suggest that flavone, which has potent influenza virus sialidase inhibitory activity, may have anti-influenza virus activity.