Scanning and transmission electron microscope studies of interkinetic nuclear migration in the cerebral vesicles of the rat
Scanning and transmission electron microscope studies of interkinetic nuclear migration in the cerebral vesicles of the rat
复制标题
大鼠脑泡核运动间迁移的扫描和透射电镜研究
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
M. Berry
中科院分区：
文献类型：
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作者：
R. M. Seymour;M. Berry
采用扫描电子显微镜（SEM）观察妊娠12、13、14天大鼠脑内运动核迁移过程中神经上皮细胞的形态和表面纹理。还制备了同龄胚胎材料的连续切片用于透射电子显微镜（TEM）。特别注意有丝分裂神经上皮细胞的 SEM 和 TEM 外观，这些细胞仅沿着神经上皮的脑室边界发生。在扫描显微照片中发现了三类形状独特的有丝分裂细胞。 (1)梨状细胞。这种类型的有丝分裂细胞的特征是存在非常长的鳍状突起，从短外部（基部）突起的尖端和肩部延伸出来。这些精细的过程被称为“有丝分裂内丝状伪足”，微绒毛存在于大多数梨状细胞的表面。 (2) 圆锥形细胞。缺乏外突，但胞体基极有大量运动内丝状伪足，周核表面富含微绒毛。 (3)球状细胞。可以将此类细胞细分为大类型和小类型。两种大小的球状细胞均不存在外部突起和微绒毛，但通常两种大小的球状细胞均不存在一些短的有丝分裂内丝状伪足，但通常在鼻极处存在一些短的有丝分裂内丝状伪足。球状群体的核周表面被粗糙的细胞和气泡状突起所修饰。
A scanning electron microscope (SEM) was used to examine the morphology and surface texture of neuroepithelial cells during interkinetic nuclear migration in the cerebral visicles of the rat at 12, 13 and 14 days of gestation. Serial sections of embryonic material of the same age were also prepared for the transmission electron microscope (TEM). Particular attention was paid to the SEM and TEM appearance of mitotic neuroepithelial cells which occur exclusively along the ventrivcular border of the neural epithelium. Three distinctly‐shaped classes of mitotic cells were recognised in sacnning micrographs. (1) Pyriform cell. This type of mitotic cells was characterised by the presence of very long, fin procsses rediating from the tip and shoulders of a short external (basal) process. These fine processes were temed “intramitoticfilopodia” Microvilli were found on the surface of most pyriform cells. (2) Conical cells. Thee lacked an external process but there were large numbers of intramototic filopodia at the basal pole of the cell body, and the perkikaryal surface was rich in microvilli. (3)Globular cells. It was possible to subdivide this class of cell into large and small types. An external process and microvilli were absent in globuar cells of both sizes, but usually a few short intramitotic filopodia were absent in globlar cells of both sizes, but usually a few short intramitotic filopodia were present at the bnasal pole. The perikaryal surfced of the globular population were reised in coarse liums and bubble‐like protrusions.