Serum Survival of Vibrio vulnificus: Role of Genotype, Capsule, Complement, Clinical Origin, and in Situ Incubation
Serum Survival of Vibrio vulnificus: Role of Genotype, Capsule, Complement, Clinical Origin, and in Situ Incubation
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创伤弧菌的血清存活：基因型、荚膜、补体、临床起源和原位培养的作用
作者：
T. Williams;M. Ayrapetyan;H. Ryan;J. Oliver
人类病原体创伤弧菌的毒力与包囊、血清补体抵抗和基因型有关。该细菌的C基因型与毒力和分离来源（临床环境）相关（>90%）。E基因型菌株与环境隔离高度相关（93%），但毒性较低。在这项研究中，我们的特点的重要性，基因型，封装，血清补体，并在原位暴露于河口水的生存的两种基因型的人血清。结果证实了C-基因型菌株暴露于人血清的上级存活能力以及补体的重要性，并揭示了缺乏包膜允许C-和E-基因型的血清杀伤。原位孵育的细胞与体外孵育的细胞反应相似，但E-环境菌株除外。有趣的是，我们的研究发现，从伤口感染中分离的E基因型细胞（通常被认为是非致病性的）表现出与毒性C基因型菌株相似的血清存活。
Virulence of the human pathogen, V. vulnificus, is associated with encapsulation, serum complement resistance, and genotype. The C-genotype of this bacterium is correlated (>90%) with virulence and with isolation source (clinical settings). E-genotype strains are highly correlated with environmental isolation (93%) but appear less virulent. In this study, we characterized the importance of genotype, encapsulation, serum complement, and in situ exposure to estuarine water on the survival of the two genotypes in human serum. Results confirmed the superior ability of C-genotype strains to survive exposure to human serum, as well as the significance of complement, and revealed that lack of capsule allowed serum killing of both C- and E-genotypes. Cells incubated in situ responded similarly to cells incubated in vitro with the exception of E-environmental strains. Interestingly, our studies found that those cells of the E-genotype, typically considered non-pathogenic, which were isolated from wound infections demonstrated serum survival similar to that of virulent, C-genotype, strains.