Digital diffraction analysis enables low-cost molecular diagnostics on a smartphone
Digital diffraction analysis enables low-cost molecular diagnostics on a smartphone
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DOI：
10.1073/pnas.1501815112
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发表时间：
2015-05-05
影响因子：
11.1
通讯作者：
Lee, Hakho
中科院分区：
文献类型：
--
作者：
Im, Hyungsoon;Castro, Cesar M.;Lee, Hakho
智能手机的广泛分布及其集成的传感器和通信功能使其成为床旁（POC）诊断的理想平台，特别是在资源有限的环境中。然而，分子诊断很难在智能手机中实现。我们在此报告了一种基于衍射的方法，使分子和细胞诊断。D3（数字衍射诊断）系统使用微珠生成独特的衍射图案，这些图案可以通过智能手机获取并由远程服务器处理。我们应用D3平台筛查宫颈标本中的癌前或癌细胞，并检测人乳头瘤病毒（HPV）DNA。D3检测试剂盒在45分钟内产生读数，并分别与金标准病理学或HPV检测显示出极好的一致性。这种方法可能具有有利的全球健康应用，其中医疗访问受限或当病理学瓶颈挑战快速诊断读数时。
The widespread distribution of smartphones, with their integrated sensors and communication capabilities, makes them an ideal platform for point-of-care (POC) diagnosis, especially in resource-limited settings. Molecular diagnostics, however, have been difficult to implement in smartphones. We herein report a diffraction-based approach that enables molecular and cellular diagnostics. The D3 (digital diffraction diagnosis) system uses microbeads to generate unique diffraction patterns which can be acquired by smartphones and processed by a remote server. We applied the D3 platform to screen for precancerous or cancerous cells in cervical specimens and to detect human papillomavirus (HPV) DNA. The D3 assay generated readouts within 45 min and showed excellent agreement with gold-standard pathology or HPV testing, respectively. This approach could have favorable global health applications where medical access is limited or when pathology bottlenecks challenge prompt diagnostic readouts.