Simultaneous yet independent regulation of actin cytoskeletal organization and translation initiation by glucose in Saccharomyces cerevisiae
Simultaneous yet independent regulation of actin cytoskeletal organization and translation initiation by glucose in Saccharomyces cerevisiae
复制标题
DOI：
10.1091/mbc.e03-12-0877
复制
发表时间：
2004-04-01
影响因子：
3.3
通讯作者：
Toh-e, A
中科院分区：
文献类型：
--
作者：
Uesono, Y;Ashe, MP;Toh-e, A
急性葡萄糖剥夺迅速但短暂地使肌动蛋白细胞骨架去极化并抑制酿酒酵母的翻译起始。无论是快速肌动蛋白去极化，也不翻译抑制葡萄糖去除发生在reg 1破坏，这是有缺陷的葡萄糖抑制，或在tpk 1（w）突变体，这有弱cAPK活性。在没有额外的葡萄糖的情况下，无论是肌动蛋白极化或翻译起始的恢复依赖于呼吸，Snf 1 p蛋白激酶，和转录因子Msn 2 p和Msn 4p。葡萄糖的再添加到葡萄糖饥饿的细胞引起肌动蛋白极化的快速恢复以及翻译起始而没有呼吸。这些结果表明，肌动蛋白极化和翻译起始的同时调节分为三个反应：1）葡萄糖去除后依赖于Reg 1 p和cAPK的快速关闭，2）葡萄糖缺乏时依赖于Snf 1 p和Msn 2 p/4p的缓慢适应，以及3）葡萄糖重新加入后快速恢复。去除葡萄糖后，翻译起始在rom 2破坏剂中被迅速抑制，这在快速肌动蛋白去极化中是有缺陷的，而快速肌动蛋白去极化发生在pop 2/caf 1破坏剂中，这在翻译起始的快速抑制中是有缺陷的。因此，翻译起始和肌动蛋白极化似乎是同时，但独立的葡萄糖剥夺调节。
Acute glucose deprivation rapidly but transiently depolarizes the actin cytoskeleton and inhibits translation initiation in Saccharomyces cerevisiae. Neither rapid actin depolarization nor translation inhibition upon glucose removal occurs in a reg1 disruptant, which is defective in glucose repression, or in the tpk1(w) mutant, which has weak cAPK activity. In the absence of additional glucose, recovery of either actin polarization or translation initiation relies upon respiration, the Snf1p protein kinase, and the transcription factors Msn2p and Msn4p. The readdition of glucose to glucose-starved cells causes a rapid recovery of actin polarization as well as translation initiation without respiration. These results indicate that the simultaneous regulation of actin polarization and translation initiation is divided into three reactions: 1) rapid shutdown depending on Reg1p and cAPK after glucose removal, 2) slow adaptation depending on Snf1p and Msn2p/4p in the absence of glucose, and 3) rapid recovery upon readdition of glucose. On glucose removal, translation initiation is rapidly inhibited in a rom2 disruptant, which is defective in rapid actin depolarization, whereas rapid actin depolarization occurs in a pop2/caf1 disruptant, which is defective in rapid inhibition of translation initiation. Thus, translation initiation and actin polarization seem to be simultaneously but independently regulated by glucose deprivation.