Right-elevated expression of charon is regulated by fluid flow in medaka Kupffer's vesicle
Right-elevated expression of charon is regulated by fluid flow in medaka Kupffer's vesicle
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DOI：
10.1111/j.1440-169x.2007.00937.x
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发表时间：
2007-06-01
影响因子：
2.5
通讯作者：
Takeda, Hiroyuki
中科院分区：
文献类型：
--
作者：
Hojo, Motoki;Takashima, Shigeo;Takeda, Hiroyuki
最近的研究表明，纤毛产生的液体流在小鼠左-右(LR)轴的建立中起着至关重要的作用。在鱼类中，库普弗氏囊泡(KV)是附着在尾部的硬骨鱼特有的球形器官，在LR轴形成过程中与小鼠的结节具有同等的作用。然而，目前还没有关于KV在流体控制下表达不对称基因的报道。在这里，我们报道了硬骨鱼KV中最早的不对称基因，Medaka Charon，及其调控。Charon是Cerberus/Dan蛋白质家族的成员，最先在斑马鱼中发现。虽然斑马鱼Charon被报道在KV中对称表达，但Medaka Charon表现出不对称表达，右侧表达更强烈。这种不对称的表达被发现受到KV流的调节，因为在具有静止纤毛或KV断裂的流动缺陷胚胎中观察到对称和上调的Charon表达。总而言之，青竹Charon是KV中LR不对称的可靠基因标记，因此，将有助于分析流体流动的下游早期步骤。
Recent studies have revealed that a cilium-generated liquid flow in the node has a crucial role in the establishment of the left-right (LR) axis in the mouse. In fish, Kupffer's vesicle (KV), a teleost-specific spherical organ attached to the tail region, is known to have an equivalent role to the mouse node during LR axis formation. However, at present, there has been no report of an asymmetric gene expressed in KV under the control of fluid flow. Here we report the earliest asymmetric gene in teleost KV, medaka charon, and its regulation. Charon is a member of the Cerberus/DAN family of proteins, first identified in zebrafish. Although zebrafish charon was reported to be symmetrically expressed in KV, medaka charon displays asymmetric expression with more intense expression on the right side. This asymmetric expression was found to be regulated by KV flow because symmetric and up-regulated charon expression was observed in flow-defective embryos with immotile cilia or disrupted KV. Taken together, medaka charon is a reliable gene marker for LR asymmetry in KV and thus, will be useful for the analysis of the early steps downstream of the fluid flow.