Circadian disruption alters mouse lung clock gene expression and lung mechanics
Circadian disruption alters mouse lung clock gene expression and lung mechanics
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DOI：
10.1152/japplphysiol.00244.2012
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发表时间：
2012-08-01
影响因子：
3.3
通讯作者：
Massaro, Donald
中科院分区：
文献类型：
--
作者：
Hadden, Helene;Soldin, Steven J.;Massaro, Donald
放大图片作者：Hadden H，Soldin SJ，Massaro D.昼夜节律紊乱改变小鼠肺生物钟基因表达和肺力学。J Appl Physiol 113：385-392，2012.首次发表于2012年6月7日; doi：10.1152/japplphysiol.00244.2012.-人类生理和行为的大多数方面都表现出由大脑中的主生物钟驱动的24小时节律，该生物钟控制着外周生物钟。肺功能和通气受昼夜节律调节并表现出昼夜节律振荡。睡眠中断会导致昼夜节律中断，在慢性肺病患者和普通人群中很常见;然而，人们对昼夜节律中断对肺部的影响知之甚少。我们测试了昼夜节律中断改变肺中时钟基因表达的假设，这与肺力学改变有关。雌性和雄性小鼠维持在12：12-h光/暗周期（对照）或暴露于模拟慢性时差反应（CJL）的变光方案4周。对照组和CJL雌性之间的气道阻力（Rn）、组织阻尼（G）和组织弹性（H）无差异。与对照组相比，CJL男性在呼气末正压（PEEP）为2和3 cmH（2）O时的Rn较低。CJL和对照组雄性之间的G、H和G/H无差异。在CJL女性中，时钟基因Bmal 1和Rev-erb α的表达降低;其阻遏物Per 2和Cry 2的表达增加。在CJL雄性中，Clock的表达降低; Per 2和Rev-erb α的表达增加。我们的结论是，昼夜节律的破坏改变了肺力学和时钟基因的表达，这样做的性别二态性的方式。
Hadden H, Soldin SJ, Massaro D. Circadian disruption alters mouse lung clock gene expression and lung mechanics. J Appl Physiol 113: 385-392, 2012. First published June 7, 2012; doi: 10.1152/japplphysiol.00244.2012.-Most aspects of human physiology and behavior exhibit 24-h rhythms driven by a master circadian clock in the brain, which synchronizes peripheral clocks. Lung function and ventilation are subject to circadian regulation and exhibit circadian oscillations. Sleep disruption, which causes circadian disruption, is common in those with chronic lung disease, and in the general population; however, little is known about the effect on the lung of circadian disruption. We tested the hypothesis circadian disruption alters expression of clock genes in the lung and that this is associated with altered lung mechanics. Female and male mice were maintained on a 12: 12-h light/dark cycle (control) or exposed for 4 wk to a shifting light regimen mimicking chronic jet lag (CJL). Airway resistance (Rn), tissue damping (G), and tissue elastance (H) did not differ between control and CJL females. Rn at positive end-expiratory pressure (PEEP) of 2 and 3 cmH(2)O was lower in CJL males compared with controls. G, H, and G/H did not differ between CJL and control males. Among CJL females, expression of clock genes, Bmal1 and Rev-erb alpha, was decreased; expression of their repressors, Per2 and Cry 2, was increased. Among CJL males, expression of Clock was decreased; Per 2 and Rev-erb alpha expression was increased. We conclude circadian disruption alters lung mechanics and clock gene expression and does so in a sexually dimorphic manner.