Study of corneal epithelial progenitor origin and the Yap1 requirement using keratin 12 lineage tracing transgenic mice.
Study of corneal epithelial progenitor origin and the Yap1 requirement using keratin 12 lineage tracing transgenic mice.
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DOI：
10.1038/srep35202
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发表时间：
2016-10-13
影响因子：
4.6
通讯作者：
Li Q
中科院分区：
文献类型：
--
作者：
Kasetti RB;Gaddipati S;Tian S;Xue L;Kao WW;Lu Q;Li Q
角膜上皮生物学的关键问题是角膜上皮干细胞维持角膜上皮稳态和伤口愈合反应的机制，以及参与的调控分子途径。关于负责角膜上皮自我更新的祖细胞群体的位置存在明显的差异。我们已经建立了一个遗传小鼠模型，以追踪成年角膜上皮稳态和伤口愈合反应期间的角膜上皮祖细胞谱系。我们的数据显示，早期角膜上皮前体细胞表达角蛋白12起源于利姆布斯，并产生了过境放大细胞，向心迁移，分化为角膜上皮细胞。我们的研究结果支持了一个模型，即角膜上皮稳态和伤口愈合主要是由来自利姆布斯的激活的角膜缘干细胞维持的，而不是来自角膜基底上皮层。在本研究中，我们进一步证明了转录辅激活因子YAP 1在角膜缘和角膜基底上皮细胞中的核表达及其在维持这些角膜上皮祖细胞体内高增殖潜力中的重要作用。
Key issues in corneal epithelium biology are the mechanism for corneal epithelium stem cells to maintain the corneal epithelial homeostasis and wound healing responses, and what are the regulatory molecular pathways involved. There are apparent discrepancies about the locations of the progenitor populations responsible for corneal epithelial self-renewal. We have developed a genetic mouse model to trace the corneal epithelial progenitor lineages during adult corneal epithelial homeostasis and wound healing response. Our data revealed that the early corneal epithelial progenitor cells expressing keratin-12 originated from limbus, and gave rise to the transit amplifying cells that migrated centripetally to differentiate into corneal epithelial cells. Our results support a model that both corneal epithelial homeostasis and wound healing are mainly maintained by the activated limbal stem cells originating form limbus, but not from the corneal basal epithelial layer. In the present study, we further demonstrated the nuclear expression of transcriptional coactivator YAP1 in the limbal and corneal basal epithelial cells and its essential role for maintaining the high proliferative potential of those corneal epithelial progenitor cells in vivo.
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影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.3791/53370
发表时间：
2015-12-18
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Amitai-Lange A;Berkowitz E;Altshuler A;Dbayat N;Nasser W;Suss-Toby E;Tiosano B;Shalom-Feuerstein R
通讯作者：
Shalom-Feuerstein R
影响因子：
1.2
作者：
Douvaras, Panagiotis;Webb, Sheila;West, John D.
通讯作者：
West, John D.
影响因子：
11.4
作者：
Lau, Allison N.;Curtis, Stephen J.;Kim, Carla F.
通讯作者：
Kim, Carla F.
影响因子：
13.7
作者：
Di Iorio, Enzo;Kaye, Stephen B.;Willoughby, Colin E.
通讯作者：
Willoughby, Colin E.