Galectin-1 knocking down in human U87 glioblastoma cells alters their gene expression pattern
Galectin-1 knocking down in human U87 glioblastoma cells alters their gene expression pattern
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DOI：
10.1016/j.bbrc.2005.07.037
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发表时间：
2005-09-16
影响因子：
3.1
通讯作者：
Kiss, R
中科院分区：
文献类型：
--
作者：
Camby, I;Decaestecker, C;Kiss, R
我们先前已经报道了（i）患有星形细胞肿瘤的患者中恶性肿瘤的进展与半乳糖凝集素-1的肿瘤水平增加相关;（ii）在体外向U87人胶质母细胞瘤细胞添加纯化的半乳糖凝集素-1增强肿瘤细胞运动性;以及（iii）相反地，通过用反义半乳糖凝集素稳定转染来敲低该细胞系中半乳糖凝集素-1的表达，1 mRNA在颅内移植到裸鼠后损害运动性并延迟死亡。我们在这里使用cDNA微阵列分析，比较稳定转染反义半乳糖凝集素-1载体对基因表达的影响，以模拟转染和野生型细胞。在631个点中，探测可能参与癌症的基因，对所有阵列的分析有效，86个基因的表达增加至少2倍。通过免疫细胞化学提供P21（waf/p1）、cullin-2、p53、ADAM-15和MAP-2的蛋白水平增加的确认。还观察到ADAM-15的表达模式和肌动蛋白应力纤维组织的主要差异。半乳糖凝集素-1表达稳定缺陷的U87细胞的运动性显著低于对照。我们的结论是，半乳糖凝集素-1的表达的稳定抑制改变了一些基因的表达，直接或间接地影响粘附，运动和侵袭的人胶质母细胞瘤细胞。(c)2005年爱思唯尔公司All rights reserved.
We have previously reported that (i) progression of malignancy in patients bearing astrocytic tumors correlates with increased tumor levels of galectin-1; (ii) in vitro addition of purified galectin-1 to U87 human glioblastoma cells enhances tumor cell motility; and (iii) conversely, knocking down galectin-1 expression in this cell line by stable transfection with antisense galectin-1 mRNA impairs motility and delays mortality after their intracranial grafting to nude mice. We here used cDNA microarray analysis to compare the effect on gene expression of stable transfection with antisense galectin-1 vector to mock-transfected and wild-type cells. Among the 631 spots probing genes potentially involved in cancer that were valid for analysis on all the arrays the expression of 86 genes was increased at least 2-fold Confirmation of increased protein levels was provided by immunocytochemistry for P21(waf/p1), cullin-2, p53, ADAM-15, and MAP-2. Major differences in the expression patterns of ADAM-15 and the actin stress fiber organization were also observed. U87 cells stably deficient for galectin-1 expression were significantly less motile than control. We conclude that the stable inhibition of galectin-1 expression alters the expression of a number of genes that either directly or indirectly influence adhesion, motility and invasion of human glioblastoma cells. (c) 2005 Elsevier Inc. All rights reserved.