GLYCOGEN, AMMONIA AND RELATED METABOLITES IN THE BRAIN DURING SEIZURES EVOKED BY METHIONINE SULPHOXIMINE 1
GLYCOGEN, AMMONIA AND RELATED METABOLITES IN THE BRAIN DURING SEIZURES EVOKED BY METHIONINE SULPHOXIMINE 1
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蛋氨酸亚磺酰亚胺 1 引起癫痫发作期间大脑中的糖原、氨和相关代谢物
DOI：
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发表时间：
1969
影响因子：
4.7
通讯作者：
D. W. Schulz
中科院分区：
文献类型：
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作者：
J. Folbergrová;J. Passonneau;O. H. Lowry;D. W. Schulz
摘要-在给予惊厥剂甲硫氨酸磺酰亚胺（MSO）后，测量小鼠脑中ATP、P-肌酸、葡萄糖、糖原、乳酸盐、谷氨酸盐和氨的水平。在癫痫发作前或癫痫发作期间均未观察到ATP和P-肌酸水平的变化。乳酸水平不变，直到癫痫发作（4-5小时），此时水平平均增加65%。葡萄糖和糖原水平逐渐增加。就在癫痫发作之前，水平分别增加了95%和62%。在癫痫发作期间，这两种物质的总量增加了130%，大脑皮层、小脑和皮层下前脑也出现了类似的变化。通过使用定量组织化学方法，发现糖原的最大增加发生在第I和III层（未分析第II和IV层）。在第V层和第VI层中发现了逐渐较小的变化，并且在皮下白色物质中没有发现任何增加。与糖原相反，葡萄糖在所有脑层和脑下白色物质中的增加程度大致相同。
Abstract— The levels of ATP, P‐creatine, glucose, glycogen, lactate, glutamate and ammonia were measured in mouse brain after administration of the convulsive agent methionine sulphoximine (MSO). No changes were observed in ATP and P‐creatine levels either before or during the seizures. Lactate levels were unchanged until the onset of seizures (4–5 hr) at which time the levels increased an average of 65 per cent. Glucose and glycogen levels increased progressively. Just before the onset of seizures the levels had increased 95 and 62 per cent, respectively. During the seizures both substances had increased a total of 130 per cent. Comparable changes were found in cerebral cortex, cerebellum and subcortical forebrain. Through the use of quantitative histochemical methods it was found that the greatest increases in glycogen occurred in layers I and III (layers II and IV were not analysed). Progressively smaller changes were found in layers V and VI and no increase at all was found in the subjacent white matter. Glucose, in contrast to glycogen, increased to about the same degree in all cerebral layers and in subjacent white matter.