Degradation of Perfluorooctane Sulfonamide by Acinetobacter Sp. M and Its Extracellular Enzymes
Degradation of Perfluorooctane Sulfonamide by Acinetobacter Sp. M and Its Extracellular Enzymes
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不动杆菌对全氟辛烷磺酰胺的降解。
DOI：
10.1002/asia.201900638
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发表时间：
2019-07-16
影响因子：
4.1
通讯作者：
Ma, Kesen
中科院分区：
文献类型：
--
作者：
Hao, Jian;Wang, Penghong;Ma, Kesen
从江苏省某污染土壤中分离到一株不动杆菌M，该菌株能有效降解全氟辛烷磺酰胺（PFOSA）。用离子色谱法检测了PFOSA降解过程中释放的氟离子，结果表明F-与不动杆菌M.通过GC分析评估，菌株M的PFOSA降解效率约为27%。结果表明，定位于不动杆菌属菌株M细胞外的酶催化PFOSA的降解。这进一步表明了一种可能的全氟辛烷磺酸降解新机制（多步骤/途径）。结果表明，不动杆菌M菌株的胞外酶优先裂解碳-碳和碳-氟键，而不是破坏碳-硫键。在2000 mg L-1 PFOSA和0.5%（v/v）吐温-20存在下，在30 ℃和pH 7.0下优化不动杆菌属菌株M的生长条件。结果表明，菌株M对PFOSA的最佳降解时间为12 h，胞外酶对PFOSA的降解率为76%。研究结果为全氟辛烷衍生物等全氟有机化合物的生物降解提供了潜在的应用前景。
The Acinetobacter sp. strain M isolated from a contaminated soil sample in Jiangsu Province of China was found to be able to degrade perfluorooctane sulfonamide (PFOSA) effectively. Fluoride anion (F-) released from PFOSA degradation was detected by ion chromatography, and showed positive correlation to the growth curve of Acinetobacter sp. strain M. The PFOSA degradation efficiency of strain M was approximately 27 %, as assessed by GC analysis. It was shown that enzymes localized outside of cells of Acinetobacter sp. strain M catalyzed the degradation of PFOSA. This further indicates a possibly new (multi-step/pathway) mechanism for PFOSA degradation. It revealed that the extracellular enzyme of the Acinetobacter strain M preferentially cleaves carbon-carbon and carbon-fluorine bonds instead of destroying the carbon-sulfur bond. The growth condition for Acinetobacter sp. strain M was optimized at 30 degrees C and pH 7.0 in the presence of 2000 mg L-1 of PFOSA and 0.5 % (v/v) of Tween-20. The optimal PFOSA degradation time was found to be 12 h, with a degradation efficiency of 76 % by extracellular enzymes in strain M as determined by GC analysis. The result may provide potential applications for biodegradition of perfluoro organic compounds, such as derivatives of perfluorooctane (C8).