Transcripts for functionally distinct isoforms of chicken GATA-5 are differentially expressed from alternative first exons
Transcripts for functionally distinct isoforms of chicken GATA-5 are differentially expressed from alternative first exons
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DOI：
10.1074/jbc.272.13.8396
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发表时间：
1997-03-28
影响因子：
4.8
通讯作者：
Burch, JBE
中科院分区：
文献类型：
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作者：
MacNeill, C;Ayres, B;Burch, JBE
我们对 cDNA 和基因组克隆的分析出乎意料地揭示了鸡 gata-5 基因从替代的第一外显子中差异表达。此外，我们表明各自的转录物经过差异处理以产生 GATA-5 两种不同亚型的 mRNA。我们之前描述的主要亚型具有两个典型的脊椎动物 GATA 因子的 CXNCX(17)CNXC 锌指，另一方面，次要亚型只有一个这样的锌指。我们表明，这种新的亚型位于转染细胞的细胞核内，并且可以与共有的 GATA 位点结合。然而，这种 GATA-5 的截短亚型在反式激活简单靶基因的能力方面受到了损害，因此在功能上不同于 GATA-5 的主要亚型。
Our analysis of cDNA and genomic clones unexpectedly revealed that the chicken gata-5 gene is differentially expressed from alternative first exons, Moreover, we show that the respective transcripts are differentially processed to yield mrnas for two distinct isoforms of GATA-5. The major isoform, which we described previously, has two CXNCX(17)CNXC zinc fingers typical of a vertebrate GATA factor, The minor isoform, on the other hand, has only one such zinc finger, We show that this novel isoform localizes within the nuclei of transfected cells and can bind to a consensus GATA site, This truncated isoform of GATA-5 is compromised in its ability to transactivate a simple target gene, however, and thus is functionally distinct from the major isoform of GATA-5.