High-throughput molecular detection of hemorrhagic fever virus threats with applications for outbreak settings
High-throughput molecular detection of hemorrhagic fever virus threats with applications for outbreak settings
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DOI：
10.1086/520601
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发表时间：
2007-11-15
影响因子：
6.4
通讯作者：
Nichol, Stuart T.
中科院分区：
文献类型：
--
作者：
Towner, Jonathan S.;Sealy, Tara K.;Nichol, Stuart T.
在过去的十几年中，人群中丝状病毒出血热的暴发频率越来越高，现在平均每1-2年发生1次流行。许多疫情规模很大（涉及150多个病例），需要国际社会迅速作出反应，帮助实施感染控制和监测。这种增加的活动，结合当今的生物恐怖主义威胁的气候，提高了对高通量方法的需求，用于在复杂的实验室设置和移动的现场实验室情况下特异性检测这些高危害病毒。以扎伊尔埃博拉病毒为例，我们在这里描述了一个高通量的协议，RNA提取和定量逆转录聚合酶链反应分析，是安全的，快速的，可靠的发展。此外，在安哥拉最近暴发的马尔堡出血热期间建立的现场实验室对> 500份标本进行正确分析，证明了该方法对暴发环境的适用性。
Within the past dozen years, outbreaks of filoviral hemorrhagic fever within the human population have been occurring with increasing frequency, with an average of 1 epidemic now occurring every 1-2 years. Many of the outbreaks have been large (involving > 150 cases), necessitating rapid responses from the international community to help implement infection control and surveillance. This increased activity, combined with today's climate of bioterrorism threats, has heightened the need for high-throughput methodologies for specific detection of these high-hazard viruses in sophisticated laboratory setups and mobile field laboratory situations. Using Zaire Ebola virus as an example, we describe here the development of a high-throughput protocol for RNA extraction and quantitative reverse-transcription polymerase chain reaction analysis that is safe, fast, and reliable. Furthermore, the applicability of this method to an outbreak setting was demonstrated by correct analysis of > 500 specimens at a field laboratory established during a recent outbreak of Marburg hemorrhagic fever in Angola.