CLASS-I MAJOR HISTOCOMPATIBILITY PROTEINS ARE AN ESSENTIAL COMPONENT OF THE SIMIAN VIRUS-40 RECEPTOR
CLASS-I MAJOR HISTOCOMPATIBILITY PROTEINS ARE AN ESSENTIAL COMPONENT OF THE SIMIAN VIRUS-40 RECEPTOR
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DOI：
10.1128/jvi.66.4.2037-2045.1992
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发表时间：
1992-04-01
影响因子：
5.4
通讯作者：
NORKIN, LC
中科院分区：
文献类型：
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作者：
BREAU, WC;ATWOOD, WJ;NORKIN, LC
由主要组织相容性复合物 (MHC) 编码的 I 类分子将内源合成的抗原肽片段呈递给细胞毒性 T 淋巴细胞。 我们在此表明这些蛋白质是猿猴病毒 40 (SV40) 细胞表面受体的重要组成部分。 首先，SV40 与细胞的结合可以被两种针对 I 类人淋巴细胞抗原 (HLA) 蛋白的单克隆抗体阻断，但不能被其他细胞表面蛋白特异性的单克隆抗体阻断。 其次，SV40 不与两种不同的人淋巴母细胞系的细胞结合，这两种细胞系不表达表面 I 类 MHC 蛋白，因为一个系中的 β-2-微球蛋白基因和另一个系中的 HLA 复合体中存在遗传缺陷。 分别用 β-2-微球蛋白和 HLA-B8 的克隆基因转染这些细胞系，恢复其表面 I 类 MHC 蛋白的表达，并导致伴随的 SV40 结合。 最后，SV40 在体外与纯化的 HLA 蛋白结合，并选择性地与细胞表面提取物中的 I 类 MHC 蛋白结合。
The class I molecules encoded by the major histocompatibility complex (MHC) present endogenously synthesized antigenic peptide fragments to cytotoxic T lymphocytes. We show here that these proteins are an essential component of the cell surface receptor for simian virus 40 (SV40). First, SV40 binding to cells can be blocked by two monoclonal antibodies against class I human lymphocyte antigen (HLA) proteins but not by monoclonal antibodies specific for other cell surface proteins. Second, SV40 does not bind to cells of two different human lymphoblastoid cell lines which do not express surface class I MHC proteins because of genetic defects in the beta-2-microglobulin gene in one line and in the HLA complex in the other. Transfection of these cell lines with cloned genes for beta-2-microglobulin and HLA-B8, respectively, restored expression of their surface class I MHC proteins and resulted in concomitant SV40 binding. Finally, SV40 binds to purified HLA proteins in vitro and selectively binds to class I MHC proteins in a cell surface extract.