Interspecific mating of the introduced bumblebee Bombus terrestris and the native Japanese bumblebee Bombus hypocrita sapporoensis results in inviable hybrids
Interspecific mating of the introduced bumblebee Bombus terrestris and the native Japanese bumblebee Bombus hypocrita sapporoensis results in inviable hybrids
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DOI：
10.1007/s00114-008-0415-7
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发表时间：
2008-10-01
影响因子：
--
通讯作者：
Tsuchida, Koji
中科院分区：
文献类型：
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作者：
Kanbe, Yuya;Okada, Ikuko;Tsuchida, Koji
大黄蜂不仅是一种有效的传粉者，而且是一种潜在的外来入侵物种，在其原生范围之外。最近，据估计，近30%的日本本土物种熊蜂和伪熊蜂的蜂王在野外与雄性熊蜂交配，这表明本土大黄蜂可能会通过与引进物种的杂交生产而遗传退化。在这项研究中，我们在实验室条件下评估了引进的白僵菌和本地的次生白僵菌之间的杂交生产。根据杂交方向不同，种间交配的卵孵化率分别为0%和8.6%，显著低于种内交配的76.9%和78.9%。利用微卫星标记进行的遗传研究表明，单倍体和二倍体个体都存在于卵期，而所有孵化的幼虫都是单倍体。此外，组织学研究表明，来自种间交配的卵在产卵后2天终止发育。这些结果强烈表明，由于交配后的隔离机制，来自种间交配的卵是不能存活的。外来传粉者的大量释放可能会导致本地熊蜂物种的数量严重下降。
The bumblebee Bombus terrestris is not only an effective pollinator, but also a potential invasive alien species outside its native range. Recently, nearly 30% of queens of the Japanese native species Bombus hypocrita sapporoensis and B. hypocrita hypocrita were estimated to copulate with B. terrestris males in the field, suggesting that indigenous bumblebees could be genetically deteriorated through hybrid production with the introduced species. In this study, we evaluated hybrid production between the introduced B. terrestris and the indigenous B. hypocrita sapporoensis under laboratory conditions. The hatching rate of eggs derived from interspecific matings was 0% and 8.6% depending on the direction of the cross, which was significantly lower than that from intraspecific matings of B. terrestris (76.9%) and B. hypocrita sapporoensis (78.9%). Genetic studies using microsatellite markers revealed that both haploid and diploid individuals were present in the egg stage, whereas all hatched larvae were haploid. In addition, histological studies revealed that eggs derived from interspecific matings terminated development 2 days after oviposition. These results strongly suggested that eggs derived from interspecific matings are inviable due to post-mating isolation mechanisms. Mass release of exotic pollinators could cause serious population declines of native bumblebee species.