Enhanced activation of the respiratory burst oxidase in neutrophils from hypertensive patients.
Enhanced activation of the respiratory burst oxidase in neutrophils from hypertensive patients.
复制标题
高血压患者中性粒细胞呼吸爆发氧化酶的激活增强。
DOI：
10.1016/0006-291x(89)92816-7
复制
发表时间：
1989
影响因子：
3.1
通讯作者：
Edon Melloni
中科院分区：
文献类型：
--
作者：
S. Pontremoli;F. Salamino;B. Sparatore;R. D. Tullio;Mauro Patrone;Alberto Tizianello;Edon Melloni
在原发性高血压患者的中性粒细胞中，由f-Met-Leu-Phe刺激引起的NADPH依赖性O 2−产生比正常血压对照受试者的中性粒细胞高3 - 4倍。与对照细胞相比，来自高血压患者的中性粒细胞对通过非刺激剂量的激动剂的引发的响应性较低，所述对照细胞在此预处理后将超氧阴离子产生增加至接近于来自高血压患者的中性粒细胞所表达的水平。当在含有膜组分和胞质辅因子的重建无细胞系统中评价O2 −产生速率时，未观察到两组受试者中性粒细胞之间NADPH氧化酶活性的差异。这些结果与以下假设一致：高血压中性粒细胞膜水平上氧化酶功能组织的差异是激动剂刺激后O 2−产生增强的原因。
In neutrophils of patients with essential hypertension the NADPH-dependent O 2− production elicited by stimulation with f-Met-Leu-Phe is three to four fold higher in comparison with neutrophils of normotensive control subjects. Neutrophils from hypertensive patients are less responsive to priming, by non-stimulating doses of the agonist, as compared to control cells, which following this pretreatment augment superoxide anion production up to levels close to those expressed by neutrophils from hypertensive patients. No difference in NADPH oxidase activity, between neutrophils from the two groups of subjects, was observed when the rate of O 2− production was evaluated in a reconstructed cell-free system containing the membrane fraction and the cytosolic cofactors. These results are consistent with the hypothesis that differences in the functional organization of the oxidase at the membrane level in neutrophils of hypertensive are responsible for the enhanced O 2− production following agonist stimulation.