A Sensitive and Flexible Assay for Determining Histone Deacetylase 1 (HDAC1) Activity.
A Sensitive and Flexible Assay for Determining Histone Deacetylase 1 (HDAC1) Activity.
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DOI：
10.1007/978-1-4939-3667-0_1
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发表时间：
2016
影响因子：
--
通讯作者：
Mei-Yi Wu;R. Wu
中科院分区：
文献类型：
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作者：
Mei-Yi Wu;R. Wu
组蛋白乙酰化和去乙酰化构成了所谓的“组蛋白密码”的一部分，并与其他翻译后修饰协同工作以决定基因的活性。组蛋白的脱乙酰化是由一类酶进行的，称为组蛋白脱乙酰酶（HDAC）。HDAC的作用被组蛋白乙酰转移酶抵消。虽然组蛋白是HDAC的最佳表征底物，但越来越多的证据也表明非组蛋白是HDAC的同样重要的底物。由于HDAC在正常生理和病理生理条件下起重要作用，因此可以可靠地检测HDAC活性并鉴定HDAC的潜在新靶点的灵敏且灵活的脱乙酰化测定是至关重要的。
Histones acetylation and deacetylation constitute part of the so-called “histone code” and work in concert with other posttranslational modifications to determine the activity of genes. Deacetylation of histone is carried out by a class of enzymes, known as histone deacetylases (HDACs). The action of HDAC is countered by histone acetyltransferases. Although histone is the best characterized substrate of HDACs, increasing evidence also indicates that non-histone proteins are equally important subtract of HDACs. Since HDACs play an important role in normal physiological and pathophysiological conditions, a sensitive and flexible deacetylation assay that can reliably detect HDAC activity and identify potential novel targets of HDACs is critical.