Recipient-derived hepatocytes in sex-mismatched liver allografts after liver transplantation: Early versus late transplant biopsies
Recipient-derived hepatocytes in sex-mismatched liver allografts after liver transplantation: Early versus late transplant biopsies
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DOI：
10.1097/01.tp.0000144055.78462.4f
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发表时间：
2004-12-15
期刊：
影响因子：
6.2
通讯作者：
Karayalcin, S
中科院分区：
文献类型：
--
作者：
Idilman, R;Erden, E;Karayalcin, S
背景移植组织中微嵌合体的存在是明确的;然而，肝移植物中植入物出现和消失的时间范围是未知的。本研究的目的是分析肝移植后性别不匹配的个体中存在的“肝源性细胞”，比较早期与晚期移植活检中“肝源性细胞再增殖”的频率，并评估“肝源性细胞再增殖”与移植物损伤严重程度之间的关系。18例接受者的石蜡包埋肝活检标本进行了审查。其中16例来自性别不匹配的受者。其余两个从具有性别匹配供体的受体获得，并用作对照。用免疫组化和荧光原位杂交双标记方法对预处理的玻片进行检测，用抗人肝细胞抗体鉴定肝细胞，用鼠抗人细胞角蛋白7鉴定导管上皮细胞，用CEPX/Y DNA探针显示X和Y染色体。使用图像分析系统对双标记玻片进行系统检查。从移植到活检的平均时间为8.1个月。16例性别不匹配的移植物受者中有11例表现出“肝细胞再生”，平均占肝细胞的2.1%。在对照活检中，没有一个细胞显示出与供体性别来源不同的核信号。早期移植活检中“肝细胞再生”的出现率和比例明显高于晚期移植活检（P
Background. The presence of microchimerism in transplanted tissues is well defined; however, the timeframe of appearance and disappearance of engraftment in liver allograft is unknown. The aims of this study were to analyze for the presence of "recipient-derived cells" in sex-mismatched individuals after liver transplantation, comparing the frequency of "recipient-derived cell repopulation" in early versus late transplant biopsies and to evaluate the relationship between "recipient-derived cell repopulation" and the severity of graft injury.Methods. Paraffin-embedded liver biopsy samples of 18 recipients were reviewed. Sixteen of them were obtained from recipients with sex-mismatched donors. The remaining two were obtained from recipients with sex-matched donors and were used as controls. Immunohistochemistry and fluorescence in situ hybridization double-labeling method were performed on pretreated slides using anti-human hepatocyte antibody to identify hepatocytes, a mouse anti-human cytokeratin-7 to identify ductal epithelial cells, and using CEPX/Y DNA probes for visualizing X and Y chromosomes. The double-labeled slides were examined systematically using an image analyzer system.Results. The mean time from transplantation to biopsy was 8.1 months. Eleven of the 16 samples obtained from recipients with sex-mismatched grafts demonstrated "recipient-derived hepatocyte repopulation," comprising a mean of 2.1% of the hepatocytes. In the control biopsies, none of the cells demonstrated different nuclear signals from the donor's sex origin. The presence and proportion of "recipient-derived hepatocyte repopulation" rate were significantly higher in early transplant biopsies than in late transplant biopsies (P